Cell Uptake and Trafficking Behavior of Non-covalent, Coiled-coil Based Polymer-Drug Conjugates

This paper reports on the cell uptake and trafficking properties of a series of non‐covalent polymer–drug conjugates. These nanomedicines are composed of a poly(N‐(2‐hydroxypropyl)methacrylamide) backbone functionalized with multiple copies of a drug. The drug moieties are attached to the polymer via a non‐covalent, so called coiled coil motif, which is formed by heterodimerization of two complementary peptide strands, one of which is attached to the polymer carrier and the other to the drug. Cytotoxicity and FACS experiments, which were carried out with model anticancer drug or fluorophore conjugates, provided insight into the cell uptake and trafficking behavior of these conjugates. The cell uptake and trafficking properties of a series of non‐covalent, coiled‐coil based polymer–drug conjugates are studied. These therapeutics consist of a biocompatible, synthetic polymer carrier modified with peptide sequences that selectively heterodimerize with a complementary peptide sequence carrying a cargo molecule. From cytotoxicity and FACS experiments insight into the cell uptake and trafficking behavior of these nanomedicines was obtained.