Photocontrol of Protein Activity Mediated by the Cleavage Reaction of a Split Intein

I want to break free: A photoactivatable split intein can be genetically fused to a protein of interest and used in potentially general ways to relay a phototrigger in a coupled fashion (see picture). The protein staphylocoagulase can be unmasked by light and thereby activates native prothrombin.

Bibliographische Detailangaben

Veröffentlicht in:	Angewandte Chemie International Edition 2011-03, Vol.50 (14), p.3249-3252
Hauptverfasser:	Binschik, Jens, Zettler, Joachim, Mootz, Henning D.
Format:	Artikel
Sprache:	eng
Schlagworte:	allosterism biological activity Blood Coagulation cleavage reactions Humans Inteins - genetics Light Molecular Structure Photochemical Processes photochemistry Plasma - metabolism protein modifications Protein Splicing
Online-Zugang:	Volltext
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