Photocontrol of Protein Activity Mediated by the Cleavage Reaction of a Split Intein

Photocontrol of Protein Activity Mediated by the Cleavage Reaction of a Split Intein

I want to break free: A photoactivatable split intein can be genetically fused to a protein of interest and used in potentially general ways to relay a phototrigger in a coupled fashion (see picture). The protein staphylocoagulase can be unmasked by light and thereby activates native prothrombin.

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Veröffentlicht in:Angewandte Chemie International Edition 2011-03, Vol.50 (14), p.3249-3252
Hauptverfasser: Binschik, Jens, Zettler, Joachim, Mootz, Henning D.
Format: Artikel
Sprache:eng
Schlagworte:
allosterism
biological activity
Blood Coagulation
cleavage reactions
Humans
Inteins - genetics
Light
Molecular Structure
Photochemical Processes
photochemistry
Plasma - metabolism
protein modifications
Protein Splicing
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Zusammenfassung:I want to break free: A photoactivatable split intein can be genetically fused to a protein of interest and used in potentially general ways to relay a phototrigger in a coupled fashion (see picture). The protein staphylocoagulase can be unmasked by light and thereby activates native prothrombin.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201007078