Differential gene expression in Poecilia vivipara exposed to diesel oil water accommodated fraction

Differential gene expression in Poecilia vivipara exposed to diesel oil water accommodated fraction

Diesel fuel is a potential contaminant of estuarine and mangrove areas, particularly because it is the main fuel used in small boats and larger vessels. The aim of this work was to identify genes differentially expressed in the liver of Poecilia vivipara (Guppy) exposed to 10% diesel fuel water acco...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Marine environmental research 2010-01, Vol.69, p.S31-S33
Hauptverfasser: Mattos, Jaco J., Siebert, Marília N., Luchmann, Karim H., Granucci, Ninna, Dorrington, Tarquin, Stoco, Patrícia H., Grisard, Edmundo C., Bainy, Afonso C.D.
Format: Artikel
Sprache:eng
Schlagworte:
Agnatha. Pisces
Animal and plant ecology
Animal, plant and microbial ecology
Animals
Biological and medical sciences
Biotransformation
Brackish
Diesel oil
Enzymes - metabolism
Fish
Freshwater
Fundamental and applied biological sciences. Psychology
Gasoline - toxicity
Gene expression
Gene Expression Profiling
Liver - metabolism
Marine
Poecilia - metabolism
Poecilia vivipara
Sea water ecosystems
SSH
Synecology
Up-Regulation
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
Vitellogenins - metabolism
Water Pollutants, Chemical - toxicity
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Diesel fuel is a potential contaminant of estuarine and mangrove areas, particularly because it is the main fuel used in small boats and larger vessels. The aim of this work was to identify genes differentially expressed in the liver of Poecilia vivipara (Guppy) exposed to 10% diesel fuel water accommodated fraction (WAF), employing the subtractive suppressive hybridization (SSH) method. The results showed 27 differentially expressed gene fragments, 12 up-regulated and 15 down-regulated. Among the up-regulated genes were CYP1A, UDPGT1a, ABCC4, Methyltransferase and Apolipoprotein A1. Down-regulated genes included Vitellogenins, C1 Inhibitor and Complement Component 3c. The identified genes are associated with different metabolic functions like biotransformation, membrane transport and immune system, indicating the susceptibility and/or molecular responses of this organism to the toxic effects elicited by diesel fuel WSF.
ISSN:0141-1136
1879-0291
DOI:10.1016/j.marenvres.2009.11.002