Quality assessment of a placental perfusion protocol

Quality assessment of a placental perfusion protocol

Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placent...

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Veröffentlicht in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2010-08, Vol.30 (1), p.138-146
Hauptverfasser: Mathiesen, Line, Mose, Tina, Mørck, Thit Juul, Nielsen, Jeanette Kolstrup Søgaard, Nielsen, Leif Kofoed, Maroun, Lisa Leth, Dziegiel, Morten Hanefeld, Larsen, Lise Grupe, Knudsen, Lisbeth Ehlert
Format: Artikel
Sprache:eng
Schlagworte:
Animal Testing Alternatives
Antipyrine
Antipyrine - pharmacokinetics
Caesarean section
Equipment Design
Female
FITC-dextran
Humans
In Vitro Techniques
Maternal-Fetal Exchange
Modular validation approach
Perfusion - instrumentation
Perfusion - methods
Perfusion - standards
Placenta - metabolism
Placental perfusion transport model
Pregnancy
Quality Control
Reproduction - drug effects
Success rate
Toxicity Tests - instrumentation
Toxicity Tests - methods
Toxicity Tests - standards
Vaginal birth
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Zusammenfassung:Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery ( n = 59, p < 0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (
ISSN:0890-6238
1873-1708
DOI:10.1016/j.reprotox.2010.01.006