Label-Free Biosensing Based on Multilayer Fluorescent Nanocomposites and a Cationic Polymeric Transducer
This study describes the preparation and characterization of a DNA sensing architecture combining the molecular recognition capabilities of a cationic conjugated polymer transducer with highly fluorescent core−shell nanoparticles (NPs). The very structure of the probe-labeled NPs and the polymer-ind...
Gespeichert in:
Veröffentlicht in: | ACS nano 2011-03, Vol.5 (3), p.1888-1896 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | This study describes the preparation and characterization of a DNA sensing architecture combining the molecular recognition capabilities of a cationic conjugated polymer transducer with highly fluorescent core−shell nanoparticles (NPs). The very structure of the probe-labeled NPs and the polymer-induced formation of NP aggregates maximize the proximity between the polymer donor and acceptor NPs that is required for optimal resonant energy transfer. Each hybridization event is signaled by a potentially large number of excited reporters following the efficient plasmon-enhanced energy transfer between target-activated polymer transducer and fluorophores located in the self-assembled core−shell aggregates, resulting in direct molecular detection of target nucleic acids at femtomolar concentrations. |
---|---|
ISSN: | 1936-0851 1936-086X |
DOI: | 10.1021/nn102776m |