STD and trNOESY NMR Study of Receptor-Ligand Interactions in Living Cancer Cells

STD and trNOESY NMR Study of Receptor-Ligand Interactions in Living Cancer Cells

Integrin antagonists: Saturation transfer difference (STD) and transferred NOE provided useful information on membrane‐bound receptor–ligand interactions in living cells in which the integrin αvβ3 is highly expressed. The epitopes of two cyclic RGD mimics were identified by STD. The different activi...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2011-03, Vol.12 (5), p.695-699
Hauptverfasser: Potenza, Donatella, Vasile, Francesca, Belvisi, Laura, Civera, Monica, Araldi, Elena M. V.
Format: Artikel
Sprache:eng
Schlagworte:
Cell Line, Tumor
Humans
Integrin alpha Chains - chemistry
Integrin alpha Chains - metabolism
Integrin beta Chains - chemistry
Integrin beta Chains - metabolism
Ligands
living cancer cells
membrane proteins
Models, Molecular
molecular modeling
Nuclear Magnetic Resonance, Biomolecular - methods
Oligopeptides - metabolism
Protein Binding
protein-ligand interactions
STD-NMR
Urinary Bladder Neoplasms - metabolism
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Zusammenfassung:Integrin antagonists: Saturation transfer difference (STD) and transferred NOE provided useful information on membrane‐bound receptor–ligand interactions in living cells in which the integrin αvβ3 is highly expressed. The epitopes of two cyclic RGD mimics were identified by STD. The different activities of these compounds were interpreted in terms of different binding modes. The experimental evidence was confirmed by docking calculations.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201000756