Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging

Red‐ily made: The first chemical synthesis of a fully elaborated bisphosphorylated triantennary M6P N‐glycan was achieved through a highly efficient late‐stage phosphorylation strategy. A human carbonic anhydrase II‐based fluorescently tagged neoglycoprotein platform was successfully constructed in order to evaluate the M6P N‐glycan‐directed protein internalization process in cell‐based assays by confocal fluorescence imaging.