Regulation of L-selectin expression by trauma-relevant cytokines

The leukocyte adhesion molecule L-selectin is important for the rolling of neutrophil granulocytes (PMN), the first step of the cascade leading to adhesion, diapedesis, and subsequent organ dysfunction. The activation of leukocytes in the posttraumatic course is assumed to be induced by different pro-inflammatory cytokines. In the present study, we investigated the effects of stimulation with pro- and anti-inflammatory cytokines on the L-selectin surface expression on PMN. Twenty patients admitted to our institution for elective operations of the lower extremities were enrolled in a prospective clinical study. For the measurement of L-selectin expression, blood was taken before the operation (0h), as well as 24h and 48h postoperatively. Without and after incubation with different pro- and anti-inflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-10), the surface expression of L-selectin was detected using an anti-L-selectin-specific antibody and flow cytometry. L-selectin surface expression without any stimulation showed a non-significant increase 48h after the operation (63.6±6.6ng/ml at 0h, 95.2±10.5ng/ml at 48h). Stimulation with TNF-α resulted in a significant decrease of L-selectin expression at 0h (25.6±2.7ng/ml; p