Genomic comparison between attenuated Chinese equine infectious anemia virus vaccine strains and their parental virulent strains

A lentiviral vaccine, live attenuated equine infectious anemia virus (EIAV) vaccine, was developed in the 1970s, and this has made tremendous contributions to the control of equine infectious anemia (EIA) in China. Four key virus strains were generated during the attenuation of the EIAV vaccine: the original Liao-Ning strain (EIAVLN₄₀), a donkey-adapted virulent strain (EIAVDV₁₁₇), a donkey-leukocyte-attenuated vaccine strain (EIAVDLV₁₂₁), and a fetal donkey dermal cell (FDD)-adapted vaccine strain (EIAVFDDV₁₃). In this study, we analyzed the proviral genomes of these four EIAV strains and found a series of consensus substitutions among these strains. These mutations provide useful information for understanding the genetic basis of EIAV attenuation. Our results suggest that multiple mutations in a variety of genes in our attenuated EIAV vaccines not only provide a basis for virulence attenuation and induction of protective immunity but also greatly reduce the risk of reversion to virulence.