Analysis of bisphenols in soft drinks by on-line solid phase extraction fast liquid chromatography–tandem mass spectrometry
In this study, an automated on-line solid-phase extraction coupled to fast liquid chromatography–tandem mass spectrometry (on-line SPE fast LC–MS/MS) method was developed for the simultaneous analysis of bisphenol A (BPA), bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPB) and bisphenol S (BPS)...
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Veröffentlicht in: | Analytica chimica acta 2011-01, Vol.683 (2), p.227-233 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | In this study, an automated on-line solid-phase extraction coupled to fast liquid chromatography–tandem mass spectrometry (on-line SPE fast LC–MS/MS) method was developed for the simultaneous analysis of bisphenol A (BPA), bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPB) and bisphenol S (BPS) in canned soft drinks without any previous sample treatment. A C18 (12
μm particle size) loading column was used for the SPE on-line preconcentration before the liquid chromatography baseline separation of bisphenol compounds using a C18 Fused-Core™ (50
mm
×
2.1
mm i.d.) column, which took less than 3
min. Gradient elution and heated electrospray were used to reduce matrix effect and improve ionization efficiency. To select the most intense and selective transitions, fragmentation studies were performed by multiple-stage mass spectrometry in an ion trap mass analyzer and tandem mass spectrometry in a triple quadrupole instrument, this latter instrument being used for quantitation in SRM mode. Quality parameters of the method were established and we obtained a simple, fast, reproducible (RSD values lower than 10%) and accurate (precision higher than 93%) method for the analysis of bisphenols in canned soft drinks at the ng
L
−1 level using matrix-matched calibration. |
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ISSN: | 0003-2670 1873-4324 |
DOI: | 10.1016/j.aca.2010.10.034 |