A Simple HPLC Method for Quantitation of Quercetin in Herbal Extracts

A reversed-phase HPLC method with UV detection was developed for the determination of quercetin. The method produced linear response over a wide concentration range, with an average accuracy of 95% and average intra- and interday variation of 0.75 and 0.3, respectively. The exactness of the method w...

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Veröffentlicht in:	Journal of AOAC International 2011, Vol.94 (1), p.100-105
Hauptverfasser:	D'MELLO, Priscilla M, JOSHI, Urmila J, SHETGIRI, Preetam P, DASGUPTA, Tarun K, DARJI, Kamil K
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chemical properties Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Composition Dietary Supplements - analysis Dietary Supplements - standards Flavonols - analysis Food industries Fundamental and applied biological sciences. Psychology Herbs High performance liquid chromatography Humans Identification and classification Materia medica, Vegetable Methods Physiological aspects Plant extracts Plant Extracts - chemistry Psidium - chemistry Quercetin Quercetin - analysis Quercetin - standards Reference Standards Vitis - chemistry
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Zusammenfassung:	A reversed-phase HPLC method with UV detection was developed for the determination of quercetin. The method produced linear response over a wide concentration range, with an average accuracy of 95% and average intra- and interday variation of 0.75 and 0.3, respectively. The exactness of the method was proven by determining the recovery rates from 50 to 150% of standard concentration, which were found within the acceptable range of 95 to 105%. The method was used for quantitation of quercetin in the extracts of Psidium guajava, Vitis vinifera, and extracts rich in quercetin and other flavonols in the flavonoid family.
ISSN:	1060-3271 1944-7922
DOI:	10.1093/jaoac/94.1.100