DARPins against a functional IgE epitope

Research highlights ▶ Binders with nanomolar affinities against the IgE heavy chain were selected from DARPin libraries using ribosome display. ▶ Anti-IgE DARPins efficiently inhibit the IgE-FcεRI interaction. ▶ Anti-IgE DARPins inhibit proinflammatory mediator release. ▶ DARPin E_29 shows higher po...

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Veröffentlicht in:	Immunology letters 2010-10, Vol.133 (2), p.78-84
Hauptverfasser:	Baumann, Michael J, Eggel, Alexander, Amstutz, Patrick, Stadler, Beda M, Vogel, Monique
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergic diseases Allergy and Immunology Animals Ankyrin Ankyrin Repeat - genetics Anti-IgE Antibodies, Anti-Idiotypic Antibodies, Monoclonal - metabolism Antibodies, Monoclonal - therapeutic use Antibodies, Monoclonal, Humanized Antibody Affinity Asthma - drug therapy Cell Line, Tumor DARPins Designed ankyrin repeat proteins Epitopes, B-Lymphocyte - immunology Functional epitope Humans Immunoglobulin Constant Regions - genetics Immunoglobulin E - genetics Immunoglobulin E - immunology Immunoglobulin E - metabolism Omalizumab Protein Binding Protein Engineering Rats Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - metabolism Recombinant Fusion Proteins - therapeutic use Transgenes - genetics
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Beschreibung
Zusammenfassung:	Research highlights ▶ Binders with nanomolar affinities against the IgE heavy chain were selected from DARPin libraries using ribosome display. ▶ Anti-IgE DARPins efficiently inhibit the IgE-FcεRI interaction. ▶ Anti-IgE DARPins inhibit proinflammatory mediator release. ▶ DARPin E_29 shows higher potency than Omalizumab in vitro.
ISSN:	0165-2478 1879-0542
DOI:	10.1016/j.imlet.2010.07.005