Expression and functional characterization of pathogenesis-related protein family 10 gene, PgPR10-2, from Panax ginseng C.A. Meyer

A full-length sequence ( PgPR10-2) of the most abundant transcript from 14-year-old ginseng EST library was isolated and cloned. PgPR10-2 gene encodes 465 bp open reading frame and its deduced protein contains 155 amino acids. Genomic DNA sequence reveals it contains two exons interrupted by one int...

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Veröffentlicht in:	Physiological and molecular plant pathology 2010-09, Vol.74 (5), p.323-329
Hauptverfasser:	Pulla, Rama Krishna, Lee, Ok Ran, In, Jun-Gyo, Kim, Yu-Jin, Senthil, Kalaiselvi, Yang, Deok-Chun
Format:	Artikel
Sprache:	eng
Schlagworte:	Abiotic stress Amino acids Biological and medical sciences Fundamental and applied biological sciences. Psychology Panax ginseng Pathogenesis-related protein Pathogens Phytopathology. Animal pests. Plant and forest protection PR-10 Ribonuclease
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Zusammenfassung:	A full-length sequence ( PgPR10-2) of the most abundant transcript from 14-year-old ginseng EST library was isolated and cloned. PgPR10-2 gene encodes 465 bp open reading frame and its deduced protein contains 155 amino acids. Genomic DNA sequence reveals it contains two exons interrupted by one intron. Expression of PgPR10-2 gene was especially abundant in roots and its transcripts showed differentially upregulated patterns against several given pathogens and abiotic stimuli. Observed enhanced ribonuclease activity and antifungal activity from tobacco transgenic lines suggest that the possible involvement of PgPR10-2 in defense-related mechanism via ribonuclease activity against biotic and abiotic stresses.
ISSN:	0885-5765 1096-1178
DOI:	10.1016/j.pmpp.2010.05.001