The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function
Abstract The aim of this study was to investigate biologic function of bone morphorgenic protein-2 (rhBMP-2) immobilized on the heparin-grafted Ti surface. Ti surfaces were first modified by 3-aminopropyltriethoxysilane (ATPES), followed by grafting of heparin. BMP-2 was then immobilized on the hepa...
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| Veröffentlicht in: | Biomaterials 2011-01, Vol.32 (2), p.366-373 |
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| creator | Kim, Sung Eun Song, Sang-Hun Yun, Young Pil Choi, Byung-Joon Kwon, Il Keun Bae, Min Soo Moon, Ho-Jin Kwon, Yong-Dae |
| description | Abstract The aim of this study was to investigate biologic function of bone morphorgenic protein-2 (rhBMP-2) immobilized on the heparin-grafted Ti surface. Ti surfaces were first modified by 3-aminopropyltriethoxysilane (ATPES), followed by grafting of heparin. BMP-2 was then immobilized on the heparin-grafted Ti surfaces. Pristine Ti and functionalized Ti surfaces were characterized by X-ray photoelectron spectroscopy (XPS), measurement of water contact angles, and protein adsorption. The biological activity of MG-63 cells on pristine and functionalized Ti surfaces was investigated by cell proliferation assays, measurement of alkaline phosphate (ALP) activity, and determination of calcium deposition. Anti-inflammatory effects were assessed by RT-PCR to measure the transcript levels of IL-6 and TNF-α. XPS revealed that heparin and BMP-2 were successfully grafted and immobilized on the Ti surfaces, respectively. In addition, Ti surfaces with BMP-2 immobilized were more hydrophilic than pristine Ti. Furthermore, BMP-2 immobilized Ti promoted significantly higher ALP activity and calcium deposition by MG-63 cells than pristine Ti. The inflammatory response was also decreased when cells were grown on heparin-grafted, BMP-2-immobilized Ti surfaces. The results of this study suggest that by grafting heparin and immobilizing BMP-2 on Ti surfaces, inflammation can be inhibited and osteoblast function promoted. |
| doi_str_mv | 10.1016/j.biomaterials.2010.09.008 |
| format | Article |
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Ti surfaces were first modified by 3-aminopropyltriethoxysilane (ATPES), followed by grafting of heparin. BMP-2 was then immobilized on the heparin-grafted Ti surfaces. Pristine Ti and functionalized Ti surfaces were characterized by X-ray photoelectron spectroscopy (XPS), measurement of water contact angles, and protein adsorption. The biological activity of MG-63 cells on pristine and functionalized Ti surfaces was investigated by cell proliferation assays, measurement of alkaline phosphate (ALP) activity, and determination of calcium deposition. Anti-inflammatory effects were assessed by RT-PCR to measure the transcript levels of IL-6 and TNF-α. XPS revealed that heparin and BMP-2 were successfully grafted and immobilized on the Ti surfaces, respectively. In addition, Ti surfaces with BMP-2 immobilized were more hydrophilic than pristine Ti. Furthermore, BMP-2 immobilized Ti promoted significantly higher ALP activity and calcium deposition by MG-63 cells than pristine Ti. The inflammatory response was also decreased when cells were grown on heparin-grafted, BMP-2-immobilized Ti surfaces. The results of this study suggest that by grafting heparin and immobilizing BMP-2 on Ti surfaces, inflammation can be inhibited and osteoblast function promoted.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2010.09.008</identifier><identifier>PMID: 20880582</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Alkaline Phosphatase - metabolism ; Animals ; Bone Morphogenetic Protein 2 - chemistry ; Bone Morphogenetic Protein 2 - immunology ; Bone Morphogenetic Protein 2 - pharmacology ; Bone morphogenic protein-2 (BMP-2) ; Calcium - metabolism ; Cell Line ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Dentistry ; Heparin ; Heparin - chemistry ; Heparin - immunology ; Heparin - pharmacology ; Humans ; Inflammation ; Interleukin-6 - genetics ; Macrophages - drug effects ; Macrophages - metabolism ; Mice ; Microscopy, Electron, Scanning ; NIH 3T3 Cells ; Osteoblasts ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; Peri-implantitis ; Photoelectron Spectroscopy ; Reverse Transcriptase Polymerase Chain Reaction ; Titanium ; Titanium - chemistry ; Tumor Necrosis Factor-alpha - genetics</subject><ispartof>Biomaterials, 2011-01, Vol.32 (2), p.366-373</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-963d2a515ffad35196512c0b10f8551dcd5d60f56394b5f2a99597ef96d028253</citedby><cites>FETCH-LOGICAL-c532t-963d2a515ffad35196512c0b10f8551dcd5d60f56394b5f2a99597ef96d028253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961210011609$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20880582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Sung Eun</creatorcontrib><creatorcontrib>Song, Sang-Hun</creatorcontrib><creatorcontrib>Yun, Young Pil</creatorcontrib><creatorcontrib>Choi, Byung-Joon</creatorcontrib><creatorcontrib>Kwon, Il Keun</creatorcontrib><creatorcontrib>Bae, Min Soo</creatorcontrib><creatorcontrib>Moon, Ho-Jin</creatorcontrib><creatorcontrib>Kwon, Yong-Dae</creatorcontrib><title>The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract The aim of this study was to investigate biologic function of bone morphorgenic protein-2 (rhBMP-2) immobilized on the heparin-grafted Ti surface. Ti surfaces were first modified by 3-aminopropyltriethoxysilane (ATPES), followed by grafting of heparin. BMP-2 was then immobilized on the heparin-grafted Ti surfaces. Pristine Ti and functionalized Ti surfaces were characterized by X-ray photoelectron spectroscopy (XPS), measurement of water contact angles, and protein adsorption. The biological activity of MG-63 cells on pristine and functionalized Ti surfaces was investigated by cell proliferation assays, measurement of alkaline phosphate (ALP) activity, and determination of calcium deposition. Anti-inflammatory effects were assessed by RT-PCR to measure the transcript levels of IL-6 and TNF-α. XPS revealed that heparin and BMP-2 were successfully grafted and immobilized on the Ti surfaces, respectively. In addition, Ti surfaces with BMP-2 immobilized were more hydrophilic than pristine Ti. Furthermore, BMP-2 immobilized Ti promoted significantly higher ALP activity and calcium deposition by MG-63 cells than pristine Ti. The inflammatory response was also decreased when cells were grown on heparin-grafted, BMP-2-immobilized Ti surfaces. The results of this study suggest that by grafting heparin and immobilizing BMP-2 on Ti surfaces, inflammation can be inhibited and osteoblast function promoted.</description><subject>Advanced Basic Science</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Bone Morphogenetic Protein 2 - chemistry</subject><subject>Bone Morphogenetic Protein 2 - immunology</subject><subject>Bone Morphogenetic Protein 2 - pharmacology</subject><subject>Bone morphogenic protein-2 (BMP-2)</subject><subject>Calcium - metabolism</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Dentistry</subject><subject>Heparin</subject><subject>Heparin - chemistry</subject><subject>Heparin - immunology</subject><subject>Heparin - pharmacology</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Interleukin-6 - genetics</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>NIH 3T3 Cells</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>Peri-implantitis</subject><subject>Photoelectron Spectroscopy</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Titanium</subject><subject>Titanium - chemistry</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks-OFCEQxjtG446rr2CIF_XQY0EPTONhE13_Jms0cT0TGgqHsRtGoE3WV_ClpTOrMV70RCi--qqKXzXNAwprClQ82a8HHyddMHk95jWD-gByDdDfaFa03_Ytl8BvNiugG9ZKQdlJcyfnPdQ7bNjt5oRB3wPv2ar5cblDgs6hKSQ64qcpDn7033XxMSyRHR508oHoYMkQA5IppsMufsbgDTmkWNCHlpFHz999aNljUiIpvujg54nkOTltMJPq5IMb9TQdbRevmAvGYdS5EDcHs8TvNrdcHQjvXZ-nzadXLy_P37QX71-_PX920RresVIH6izTnHLntO04lYJTZmCg4HrOqTWWWwGOi05uBu6YlpLLLTopLLCe8e60eXj0re1_nTEXNflscBx1wDhnVV3EVnCAfyspo0x0fVeVT49Kk2LOCZ06JD_pdKUoqIWa2qs_qamFmgKpKrWafP-6zDxMaH-n_sJUBS-OAqzf8s1jUtl4DAatT5WcstH_X52zv2zM6CtHPX7BK8z7OKew5FCVmQL1cdmfZX1o3RwqQHY_AUoMxT4</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Kim, Sung Eun</creator><creator>Song, Sang-Hun</creator><creator>Yun, Young Pil</creator><creator>Choi, Byung-Joon</creator><creator>Kwon, Il Keun</creator><creator>Bae, Min Soo</creator><creator>Moon, Ho-Jin</creator><creator>Kwon, Yong-Dae</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110101</creationdate><title>The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function</title><author>Kim, Sung Eun ; Song, Sang-Hun ; Yun, Young Pil ; Choi, Byung-Joon ; Kwon, Il Keun ; Bae, Min Soo ; Moon, Ho-Jin ; Kwon, Yong-Dae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-963d2a515ffad35196512c0b10f8551dcd5d60f56394b5f2a99597ef96d028253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Advanced Basic Science</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Bone Morphogenetic Protein 2 - chemistry</topic><topic>Bone Morphogenetic Protein 2 - immunology</topic><topic>Bone Morphogenetic Protein 2 - pharmacology</topic><topic>Bone morphogenic protein-2 (BMP-2)</topic><topic>Calcium - metabolism</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Dentistry</topic><topic>Heparin</topic><topic>Heparin - chemistry</topic><topic>Heparin - immunology</topic><topic>Heparin - pharmacology</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Interleukin-6 - genetics</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning</topic><topic>NIH 3T3 Cells</topic><topic>Osteoblasts</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - metabolism</topic><topic>Peri-implantitis</topic><topic>Photoelectron Spectroscopy</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Titanium</topic><topic>Titanium - chemistry</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Sung Eun</creatorcontrib><creatorcontrib>Song, Sang-Hun</creatorcontrib><creatorcontrib>Yun, Young Pil</creatorcontrib><creatorcontrib>Choi, Byung-Joon</creatorcontrib><creatorcontrib>Kwon, Il Keun</creatorcontrib><creatorcontrib>Bae, Min Soo</creatorcontrib><creatorcontrib>Moon, Ho-Jin</creatorcontrib><creatorcontrib>Kwon, Yong-Dae</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Sung Eun</au><au>Song, Sang-Hun</au><au>Yun, Young Pil</au><au>Choi, Byung-Joon</au><au>Kwon, Il Keun</au><au>Bae, Min Soo</au><au>Moon, Ho-Jin</au><au>Kwon, Yong-Dae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>32</volume><issue>2</issue><spage>366</spage><epage>373</epage><pages>366-373</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract The aim of this study was to investigate biologic function of bone morphorgenic protein-2 (rhBMP-2) immobilized on the heparin-grafted Ti surface. Ti surfaces were first modified by 3-aminopropyltriethoxysilane (ATPES), followed by grafting of heparin. BMP-2 was then immobilized on the heparin-grafted Ti surfaces. Pristine Ti and functionalized Ti surfaces were characterized by X-ray photoelectron spectroscopy (XPS), measurement of water contact angles, and protein adsorption. The biological activity of MG-63 cells on pristine and functionalized Ti surfaces was investigated by cell proliferation assays, measurement of alkaline phosphate (ALP) activity, and determination of calcium deposition. Anti-inflammatory effects were assessed by RT-PCR to measure the transcript levels of IL-6 and TNF-α. XPS revealed that heparin and BMP-2 were successfully grafted and immobilized on the Ti surfaces, respectively. In addition, Ti surfaces with BMP-2 immobilized were more hydrophilic than pristine Ti. Furthermore, BMP-2 immobilized Ti promoted significantly higher ALP activity and calcium deposition by MG-63 cells than pristine Ti. The inflammatory response was also decreased when cells were grown on heparin-grafted, BMP-2-immobilized Ti surfaces. The results of this study suggest that by grafting heparin and immobilizing BMP-2 on Ti surfaces, inflammation can be inhibited and osteoblast function promoted.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>20880582</pmid><doi>10.1016/j.biomaterials.2010.09.008</doi><tpages>8</tpages></addata></record> |
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| subjects | Advanced Basic Science Alkaline Phosphatase - metabolism Animals Bone Morphogenetic Protein 2 - chemistry Bone Morphogenetic Protein 2 - immunology Bone Morphogenetic Protein 2 - pharmacology Bone morphogenic protein-2 (BMP-2) Calcium - metabolism Cell Line Cell Line, Tumor Cell Proliferation - drug effects Dentistry Heparin Heparin - chemistry Heparin - immunology Heparin - pharmacology Humans Inflammation Interleukin-6 - genetics Macrophages - drug effects Macrophages - metabolism Mice Microscopy, Electron, Scanning NIH 3T3 Cells Osteoblasts Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - metabolism Peri-implantitis Photoelectron Spectroscopy Reverse Transcriptase Polymerase Chain Reaction Titanium Titanium - chemistry Tumor Necrosis Factor-alpha - genetics |
| title | The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function |
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