Modification of transmembrane and GPI-anchored proteins on living cells by efficient protein trans-splicing using the Npu DnaE intein

The naturally split Npu DnaE intein can be used for ligation of an exogenous polypeptide to membrane proteins on living cells. No reducing agents or other factors are required. The approach is rapid and virtually traceless, because the intein removes itself during the reaction.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (11), p.3063-3065
Hauptverfasser:	Dhar, Tulika, Mootz, Henning D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Cell Membrane - metabolism Chemical communication DNA Polymerase III - genetics DNA Polymerase III - metabolism GPI-Linked Proteins - analysis GPI-Linked Proteins - genetics GPI-Linked Proteins - metabolism Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Inteins Mice Nostoc - enzymology Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Trans-Splicing
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Beschreibung
Zusammenfassung:	The naturally split Npu DnaE intein can be used for ligation of an exogenous polypeptide to membrane proteins on living cells. No reducing agents or other factors are required. The approach is rapid and virtually traceless, because the intein removes itself during the reaction.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c0cc04172f