A Novel Material Incorporation Technique for Medaka (Oryzias latipes) Eggs Using Nanosecond Pulsed Electric Fields

The effects of nanosecond pulsed electric fields (nsPEFs) on medaka eggs were examined. Although embryogenesis was not affected by nsPEF treatment alone, significant harmful effects were observed when the eggs were treated with nsPEFs in the presence of cycloheximide in the outer solution. Nanosecon...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (6), p.1279-1282
Hauptverfasser:	TOMINAGA, Nobuaki, KONO, Susumu, YAMAGUCHI, Akemi, AKIYAMA, Hidenori, ARIZONO, Koji
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biochemistry Biological and medical sciences Biotechnology Blumeline line generator Cycloheximide - pharmacology Eggs Electric fields Electricity electroporation embryogenesis Embryonic Development - drug effects Fertilization - drug effects Fundamental and applied biological sciences. Psychology medaka Membranes Nanocomposites Nanomaterials nanosecond pulsed electric field Nanostructure Oryzias - embryology Oryzias - physiology Oryzias latipes Ovum - drug effects Ovum - physiology Permeability Time Factors
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Zusammenfassung:	The effects of nanosecond pulsed electric fields (nsPEFs) on medaka eggs were examined. Although embryogenesis was not affected by nsPEF treatment alone, significant harmful effects were observed when the eggs were treated with nsPEFs in the presence of cycloheximide in the outer solution. Nanosecond PEF treatment affected the permeability of both the egg envelope (chorion) and the cell membrane, which resulted in intracellular incorporation of cycloheximide.
ISSN:	0916-8451 1347-6947 1347-6947
DOI:	10.1271/bbb.90958