MALDI-TOF Mass Spectrometric Analysis of Enzyme Activity and Lectin Trapping on an Array of N-Glycans
Active arrays: Complex lipid‐tagged oligosaccharides, including large multiantennary species, can be efficiently immobilized on self‐assembled monolayers of alkyl mercaptans (see picture). These arrays can be used to follow the action of a galactosyltransferase (GalT) and a hydrolase. The utility of...
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Veröffentlicht in: | Angewandte Chemie (International ed.) 2011-02, Vol.50 (8), p.1801-1804 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Active arrays: Complex lipid‐tagged oligosaccharides, including large multiantennary species, can be efficiently immobilized on self‐assembled monolayers of alkyl mercaptans (see picture). These arrays can be used to follow the action of a galactosyltransferase (GalT) and a hydrolase. The utility of the system for the selective trapping and identification of a lectin from a complex mixture was also demonstrated. |
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ISSN: | 1433-7851 1521-3773 |
DOI: | 10.1002/anie.201006304 |