Simultaneous measurement of ATPase and superprecipitation of myosin B
A combination is described of two laboratory instruments which simultaneously measures the extent of increasing turbidity and an accompanying enzymic reaction. The instrument consists of a Beckman DU spectrophotometer (serving as a densitometer) and Radiometer Titrator (serving as a pH-stat) which a...
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Veröffentlicht in: | Analytical biochemistry 1968, Vol.25 (1), p.136-145 |
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Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A combination is described of two laboratory instruments which simultaneously measures the extent of increasing turbidity and an accompanying enzymic reaction. The instrument consists of a Beckman DU spectrophotometer (serving as a densitometer) and Radiometer Titrator (serving as a pH-stat) which are made to record the progress of the two phenomena on a Texas Instrument dual-channel recorder. The two concomitant phenomena, in this instance, are the superprecipitation of myosin B and the accompanying enzymic dephosphorylation of ATP. Experimental results are presented to demonstrate the efficiency of the instrumentation for quantitating these phenomena. |
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ISSN: | 0003-2697 1096-0309 |
DOI: | 10.1016/0003-2697(68)90087-0 |