Simultaneous measurement of ATPase and superprecipitation of myosin B

A combination is described of two laboratory instruments which simultaneously measures the extent of increasing turbidity and an accompanying enzymic reaction. The instrument consists of a Beckman DU spectrophotometer (serving as a densitometer) and Radiometer Titrator (serving as a pH-stat) which a...

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Veröffentlicht in:Analytical biochemistry 1968, Vol.25 (1), p.136-145
Hauptverfasser: Evans, T.C., Bowen, William J.
Format: Artikel
Sprache:eng
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Zusammenfassung:A combination is described of two laboratory instruments which simultaneously measures the extent of increasing turbidity and an accompanying enzymic reaction. The instrument consists of a Beckman DU spectrophotometer (serving as a densitometer) and Radiometer Titrator (serving as a pH-stat) which are made to record the progress of the two phenomena on a Texas Instrument dual-channel recorder. The two concomitant phenomena, in this instance, are the superprecipitation of myosin B and the accompanying enzymic dephosphorylation of ATP. Experimental results are presented to demonstrate the efficiency of the instrumentation for quantitating these phenomena.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(68)90087-0