Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system
A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed...
Gespeichert in:
| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-02, Vol.879 (5), p.457-460 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 460 |
|---|---|
| container_issue | 5 |
| container_start_page | 457 |
| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| container_volume | 879 |
| creator | Soares, Paulo A.G. Nascimento, Cynthia O. Porto, Tatiana S. Correia, Maria T.S. Porto, Ana L.F. Carneiro-da-Cunha, Maria G. |
| description | A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of
Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis. |
| doi_str_mv | 10.1016/j.jchromb.2010.12.030 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_850563238</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1570023210007865</els_id><sourcerecordid>850563238</sourcerecordid><originalsourceid>FETCH-LOGICAL-c480t-8396b4678b89cce91a5d83850cd5642ad9a9df86f834119de67c23105d7af793</originalsourceid><addsrcrecordid>eNqFkcuKFDEUhoMoztj6CGo2optqc6ncViLNOAoDDjiCu3A6lcykqaq0SdXI7HwH39AnMUW3utNNEsJ3zvk4P0JPKVlTQuXr3XrnbnIatmtGlj-2JpzcQ6dUK95wJb_cr2-hSEMYZyfoUSk7Qqgiij9EJ4wyIYkRp8hezjmG6GCKacQpYMC9d1MccajN8QZGuIU-AvZjiSHlIRY8lzhe48uz85_ff7g4ZZg8hq-zT3PB07fU7G-geFzuyuSHx-hBgL74J8d7ha7enV1t3jcXH88_bN5eNK7VZGo0N3LbSqW32jjnDQXRaa4FcZ2QLYPOgOmClkHzllLTeakc45SITkFQhq_Qy0PbfU7VpEy2ijrf9zAuWrZ2EpIzriv56p8klYoy3i7HCokD6nIqJftg9zkOkO8sJXYJwe7sMQS7hGApszWEWvfsOGLeDr77U_V76xV4cQSgOOhDhtHF8pfjWilOFtfnBy5AsnCdK_P5U50kCCFKUrMovjkQvu72Nvpsi4t-dL6LucZouxT_I_sLpImxtw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1671234712</pqid></control><display><type>article</type><title>Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Soares, Paulo A.G. ; Nascimento, Cynthia O. ; Porto, Tatiana S. ; Correia, Maria T.S. ; Porto, Ana L.F. ; Carneiro-da-Cunha, Maria G.</creator><creatorcontrib>Soares, Paulo A.G. ; Nascimento, Cynthia O. ; Porto, Tatiana S. ; Correia, Maria T.S. ; Porto, Ana L.F. ; Carneiro-da-Cunha, Maria G.</creatorcontrib><description>A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of
Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2010.12.030</identifier><identifier>PMID: 21256095</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analysis of Variance ; Analytical, structural and metabolic biochemistry ; Aqueous two-phase system ; Biological and medical sciences ; Canavalia - chemistry ; Canavalia ensiformis ; Chemical Fractionation - methods ; Chromatography ; Citrates ; Citric Acid - chemistry ; concanavalin A ; Concanavalin A - chemistry ; Concanavalin A - isolation & purification ; Downstream processing ; Electrophoresis, Polyacrylamide Gel ; Extraction ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Hydrogen-Ion Concentration ; Lectin purification ; Lectins ; Medical sciences ; Molecular Weight ; Partitions ; Pharmacology. Drug treatments ; Plant Extracts - chemistry ; polyacrylamide gel electrophoresis ; polyethylene glycol ; Polyethylene Glycols - chemistry ; Purification ; Seeds ; Seeds - chemistry</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-02, Vol.879 (5), p.457-460</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-8396b4678b89cce91a5d83850cd5642ad9a9df86f834119de67c23105d7af793</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2010.12.030$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23877308$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21256095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soares, Paulo A.G.</creatorcontrib><creatorcontrib>Nascimento, Cynthia O.</creatorcontrib><creatorcontrib>Porto, Tatiana S.</creatorcontrib><creatorcontrib>Correia, Maria T.S.</creatorcontrib><creatorcontrib>Porto, Ana L.F.</creatorcontrib><creatorcontrib>Carneiro-da-Cunha, Maria G.</creatorcontrib><title>Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of
Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis.</description><subject>Analysis</subject><subject>Analysis of Variance</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Aqueous two-phase system</subject><subject>Biological and medical sciences</subject><subject>Canavalia - chemistry</subject><subject>Canavalia ensiformis</subject><subject>Chemical Fractionation - methods</subject><subject>Chromatography</subject><subject>Citrates</subject><subject>Citric Acid - chemistry</subject><subject>concanavalin A</subject><subject>Concanavalin A - chemistry</subject><subject>Concanavalin A - isolation & purification</subject><subject>Downstream processing</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Extraction</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lectin purification</subject><subject>Lectins</subject><subject>Medical sciences</subject><subject>Molecular Weight</subject><subject>Partitions</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - chemistry</subject><subject>polyacrylamide gel electrophoresis</subject><subject>polyethylene glycol</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Purification</subject><subject>Seeds</subject><subject>Seeds - chemistry</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuKFDEUhoMoztj6CGo2optqc6ncViLNOAoDDjiCu3A6lcykqaq0SdXI7HwH39AnMUW3utNNEsJ3zvk4P0JPKVlTQuXr3XrnbnIatmtGlj-2JpzcQ6dUK95wJb_cr2-hSEMYZyfoUSk7Qqgiij9EJ4wyIYkRp8hezjmG6GCKacQpYMC9d1MccajN8QZGuIU-AvZjiSHlIRY8lzhe48uz85_ff7g4ZZg8hq-zT3PB07fU7G-geFzuyuSHx-hBgL74J8d7ha7enV1t3jcXH88_bN5eNK7VZGo0N3LbSqW32jjnDQXRaa4FcZ2QLYPOgOmClkHzllLTeakc45SITkFQhq_Qy0PbfU7VpEy2ijrf9zAuWrZ2EpIzriv56p8klYoy3i7HCokD6nIqJftg9zkOkO8sJXYJwe7sMQS7hGApszWEWvfsOGLeDr77U_V76xV4cQSgOOhDhtHF8pfjWilOFtfnBy5AsnCdK_P5U50kCCFKUrMovjkQvu72Nvpsi4t-dL6LucZouxT_I_sLpImxtw</recordid><startdate>20110215</startdate><enddate>20110215</enddate><creator>Soares, Paulo A.G.</creator><creator>Nascimento, Cynthia O.</creator><creator>Porto, Tatiana S.</creator><creator>Correia, Maria T.S.</creator><creator>Porto, Ana L.F.</creator><creator>Carneiro-da-Cunha, Maria G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20110215</creationdate><title>Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system</title><author>Soares, Paulo A.G. ; Nascimento, Cynthia O. ; Porto, Tatiana S. ; Correia, Maria T.S. ; Porto, Ana L.F. ; Carneiro-da-Cunha, Maria G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-8396b4678b89cce91a5d83850cd5642ad9a9df86f834119de67c23105d7af793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analysis</topic><topic>Analysis of Variance</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Aqueous two-phase system</topic><topic>Biological and medical sciences</topic><topic>Canavalia - chemistry</topic><topic>Canavalia ensiformis</topic><topic>Chemical Fractionation - methods</topic><topic>Chromatography</topic><topic>Citrates</topic><topic>Citric Acid - chemistry</topic><topic>concanavalin A</topic><topic>Concanavalin A - chemistry</topic><topic>Concanavalin A - isolation & purification</topic><topic>Downstream processing</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Extraction</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lectin purification</topic><topic>Lectins</topic><topic>Medical sciences</topic><topic>Molecular Weight</topic><topic>Partitions</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - chemistry</topic><topic>polyacrylamide gel electrophoresis</topic><topic>polyethylene glycol</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Purification</topic><topic>Seeds</topic><topic>Seeds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soares, Paulo A.G.</creatorcontrib><creatorcontrib>Nascimento, Cynthia O.</creatorcontrib><creatorcontrib>Porto, Tatiana S.</creatorcontrib><creatorcontrib>Correia, Maria T.S.</creatorcontrib><creatorcontrib>Porto, Ana L.F.</creatorcontrib><creatorcontrib>Carneiro-da-Cunha, Maria G.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soares, Paulo A.G.</au><au>Nascimento, Cynthia O.</au><au>Porto, Tatiana S.</au><au>Correia, Maria T.S.</au><au>Porto, Ana L.F.</au><au>Carneiro-da-Cunha, Maria G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2011-02-15</date><risdate>2011</risdate><volume>879</volume><issue>5</issue><spage>457</spage><epage>460</epage><pages>457-460</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of
Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21256095</pmid><doi>10.1016/j.jchromb.2010.12.030</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-02, Vol.879 (5), p.457-460 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_850563238 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analysis Analysis of Variance Analytical, structural and metabolic biochemistry Aqueous two-phase system Biological and medical sciences Canavalia - chemistry Canavalia ensiformis Chemical Fractionation - methods Chromatography Citrates Citric Acid - chemistry concanavalin A Concanavalin A - chemistry Concanavalin A - isolation & purification Downstream processing Electrophoresis, Polyacrylamide Gel Extraction Fundamental and applied biological sciences. Psychology General pharmacology Hydrogen-Ion Concentration Lectin purification Lectins Medical sciences Molecular Weight Partitions Pharmacology. Drug treatments Plant Extracts - chemistry polyacrylamide gel electrophoresis polyethylene glycol Polyethylene Glycols - chemistry Purification Seeds Seeds - chemistry |
| title | Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T23%3A33%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20of%20a%20lectin%20from%20Canavalia%20ensiformis%20using%20PEG%E2%80%93citrate%20aqueous%20two-phase%20system&rft.jtitle=Journal%20of%20chromatography.%20B,%20Analytical%20technologies%20in%20the%20biomedical%20and%20life%20sciences&rft.au=Soares,%20Paulo%20A.G.&rft.date=2011-02-15&rft.volume=879&rft.issue=5&rft.spage=457&rft.epage=460&rft.pages=457-460&rft.issn=1570-0232&rft.eissn=1873-376X&rft_id=info:doi/10.1016/j.jchromb.2010.12.030&rft_dat=%3Cproquest_cross%3E850563238%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1671234712&rft_id=info:pmid/21256095&rft_els_id=S1570023210007865&rfr_iscdi=true |