Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system

Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system

A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed...

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Veröffentlicht in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-02, Vol.879 (5), p.457-460
Hauptverfasser: Soares, Paulo A.G., Nascimento, Cynthia O., Porto, Tatiana S., Correia, Maria T.S., Porto, Ana L.F., Carneiro-da-Cunha, Maria G.
Format: Artikel
Sprache:eng
Schlagworte:
Analysis
Analysis of Variance
Analytical, structural and metabolic biochemistry
Aqueous two-phase system
Biological and medical sciences
Canavalia - chemistry
Canavalia ensiformis
Chemical Fractionation - methods
Chromatography
Citrates
Citric Acid - chemistry
concanavalin A
Concanavalin A - chemistry
Concanavalin A - isolation & purification
Downstream processing
Electrophoresis, Polyacrylamide Gel
Extraction
Fundamental and applied biological sciences. Psychology
General pharmacology
Hydrogen-Ion Concentration
Lectin purification
Lectins
Medical sciences
Molecular Weight
Partitions
Pharmacology. Drug treatments
Plant Extracts - chemistry
polyacrylamide gel electrophoresis
polyethylene glycol
Polyethylene Glycols - chemistry
Purification
Seeds
Seeds - chemistry
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Beschreibung
Zusammenfassung:A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2010.12.030