Preparation of follicle stimulating hormone from sheep pituitary glands

Preparation of follicle stimulating hormone from sheep pituitary glands

A method is reported for the preparation of highly purified follicle stimulating hormone from sheep pituitary glands. The FSH was prepared from extracts of the fresh glands by chromatography on Sephadex G-100 and DEAE-Sephadex. The FSH obtained was shown by the Steelman-Pohley assay to be about 44 t...

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Veröffentlicht in:Biochemical and biophysical research communications 1965-10, Vol.21 (2), p.120-125
Hauptverfasser: Hashimoto, C., McShan, W.H., Meyer, R.K.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Chemical Phenomena
Chemistry
Electrophoresis
Follicle Stimulating Hormone
In Vitro Techniques
Pituitary Gland
Sheep
Ultracentrifugation
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Zusammenfassung:A method is reported for the preparation of highly purified follicle stimulating hormone from sheep pituitary glands. The FSH was prepared from extracts of the fresh glands by chromatography on Sephadex G-100 and DEAE-Sephadex. The FSH obtained was shown by the Steelman-Pohley assay to be about 44 times as active as the standard FSH preparation (NIH-FSH-S1). Physicochemical studies indicate that the FSH preparation is essentially homogeneous and the molecular weight is approximately 33,000.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(65)90096-3