Polyacrylamide gel electrophoresis: A simple system using gel columns
A polyacrylamide gel electrophoretic system based on the use of 2 1 2 - in . gel columns with a tris-glycine buffer system is described. It features relatively low voltage and amperage conditions with high migration rates, and operates at room temperature. Equipment of electrophoresis for destaining...
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Veröffentlicht in: | Analytical biochemistry 1965-01, Vol.13 (2), p.294-304 |
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container_title | Analytical biochemistry |
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creator | Matson, Charles F. |
description | A polyacrylamide gel electrophoretic system based on the use of
2
1
2
-
in
. gel columns with a tris-glycine buffer system is described. It features relatively low voltage and amperage conditions with high migration rates, and operates at room temperature. Equipment of electrophoresis for destaining and for scanning the developed patterns is described. This system is adapted for easy, simultaneous handling of large numbers of gel columns. |
doi_str_mv | 10.1016/0003-2697(65)90198-3 |
format | Article |
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. gel columns with a tris-glycine buffer system is described. It features relatively low voltage and amperage conditions with high migration rates, and operates at room temperature. Equipment of electrophoresis for destaining and for scanning the developed patterns is described. This system is adapted for easy, simultaneous handling of large numbers of gel columns.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(65)90198-3</identifier><identifier>PMID: 5863686</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acrylic Resins ; Electrophoresis - instrumentation</subject><ispartof>Analytical biochemistry, 1965-01, Vol.13 (2), p.294-304</ispartof><rights>1965</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(65)90198-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5863686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matson, Charles F.</creatorcontrib><title>Polyacrylamide gel electrophoresis: A simple system using gel columns</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A polyacrylamide gel electrophoretic system based on the use of
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. gel columns with a tris-glycine buffer system is described. It features relatively low voltage and amperage conditions with high migration rates, and operates at room temperature. Equipment of electrophoresis for destaining and for scanning the developed patterns is described. This system is adapted for easy, simultaneous handling of large numbers of gel columns.</description><subject>Acrylic Resins</subject><subject>Electrophoresis - instrumentation</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1965</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRS0EKqXwByBlhWARsOt4ErNAqqrykCrBAtaW40yLkfPATpDy9yRtxWoW98zoziHkktE7RhncU0p5PAeZ3oC4lZTJLOZHZMqohJhyKo_J9B85JWchfFPKWCJgQiYiAw4ZTMnqvXa9Nr53urQFRlt0ETo0ra-br9pjsOEhWkTBlo3DKPShxTLqgq22O9TUriurcE5ONtoFvDjMGfl8Wn0sX-L12_PrcrGOcQ6sjbnIE5ZmjEmd5EakoPkGaY6pSYUAWYzVEaXeCGC5kUYDpCkzKcV5jsMin5Hr_d3G1z8dhlaVNhh0TldYd0FlgnJIZDKAVwewy0ssVONtqX2vDn8P-eM-x6Htr0WvgrFYGSysH55XRW0Vo2r0rEaJapSoQKidZ8X5H9RjboY</recordid><startdate>19650101</startdate><enddate>19650101</enddate><creator>Matson, Charles F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19650101</creationdate><title>Polyacrylamide gel electrophoresis: A simple system using gel columns</title><author>Matson, Charles F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e261t-35b4178119a4bc576a3fe0be7c75569d1096ee9af561bc9ca66771c70e2be1783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1965</creationdate><topic>Acrylic Resins</topic><topic>Electrophoresis - instrumentation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matson, Charles F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matson, Charles F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyacrylamide gel electrophoresis: A simple system using gel columns</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1965-01-01</date><risdate>1965</risdate><volume>13</volume><issue>2</issue><spage>294</spage><epage>304</epage><pages>294-304</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A polyacrylamide gel electrophoretic system based on the use of
2
1
2
-
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. gel columns with a tris-glycine buffer system is described. It features relatively low voltage and amperage conditions with high migration rates, and operates at room temperature. Equipment of electrophoresis for destaining and for scanning the developed patterns is described. This system is adapted for easy, simultaneous handling of large numbers of gel columns.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5863686</pmid><doi>10.1016/0003-2697(65)90198-3</doi><tpages>11</tpages></addata></record> |
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language | eng |
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source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | Acrylic Resins Electrophoresis - instrumentation |
title | Polyacrylamide gel electrophoresis: A simple system using gel columns |
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