Polyacrylamide gel electrophoresis: A simple system using gel columns
A polyacrylamide gel electrophoretic system based on the use of 2 1 2 - in . gel columns with a tris-glycine buffer system is described. It features relatively low voltage and amperage conditions with high migration rates, and operates at room temperature. Equipment of electrophoresis for destaining...
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| Veröffentlicht in: | Analytical biochemistry 1965-01, Vol.13 (2), p.294-304 |
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| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | A polyacrylamide gel electrophoretic system based on the use of
2
1
2
-
in
. gel columns with a tris-glycine buffer system is described. It features relatively low voltage and amperage conditions with high migration rates, and operates at room temperature. Equipment of electrophoresis for destaining and for scanning the developed patterns is described. This system is adapted for easy, simultaneous handling of large numbers of gel columns. |
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| ISSN: | 0003-2697 1096-0309 |
| DOI: | 10.1016/0003-2697(65)90198-3 |