HYALURONIDASE ACTIVITY OF ALVEOLAR MACROPHAGES

Hyaluronidase activity was detected in preparations of isolated rabbit lung alveolar macrophages. The enzyme manifested a pH optimum of 3.9 with no activity detected above pH 5. Analysis of reaction products isolated from digests containing low enzyme concentrations revealed the production of large...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The journal of histochemistry and cytochemistry 1968-11, Vol.16 (11), p.688-692
Hauptverfasser: GOGGINS, JOHN F, LAZARUS, GERALD S, FULLMER, HAROLD M
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Hyaluronidase activity was detected in preparations of isolated rabbit lung alveolar macrophages. The enzyme manifested a pH optimum of 3.9 with no activity detected above pH 5. Analysis of reaction products isolated from digests containing low enzyme concentrations revealed the production of large oligosaccharides from hyaluronic acid without the liberation of free N-acetylglucosamine or glucuronic acid. On the other hand, smaller oligosaccharides and free N-acetylglucosamine were obtained from digests with high concentrations of the crude enzyme. These data in conjunction with the findings of others indicate that macrophages contain hyaluronidase and other enzymes active against polymerized hyaluronic acid and its digestion products.
ISSN:0022-1554
1551-5044
DOI:10.1177/16.11.688