Synthesis of exocellular phosphomannan by protoplasts of Hansenula holstii
The biosynthesis of exocellular phosphomannan has been demonstrated with protoplasts of Hansenula holstii NRRL Y-2448. The protoplasts were produced by the snail-gut enzyme, and incubation of the protoplasts with labelled glucose and phosphate led to the formation of an exocellular product which was...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1968-01, Vol.126 (3), p.764-770 |
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| container_issue | 3 |
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| container_title | Archives of biochemistry and biophysics |
| container_volume | 126 |
| creator | Kozak, L.P. Bretthauer, R.K. |
| description | The biosynthesis of exocellular phosphomannan has been demonstrated with protoplasts of
Hansenula holstii NRRL Y-2448. The protoplasts were produced by the snail-gut enzyme, and incubation of the protoplasts with labelled glucose and phosphate led to the formation of an exocellular product which was characterized by its hydrolytic properties at pH 2.5. The products of the hydrolyzed polymer had the same Chromatographie characteristics on Sephadex and on paper as the authentic phosphomannan added as carrier. |
| doi_str_mv | 10.1016/0003-9861(68)90469-4 |
| format | Article |
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Hansenula holstii NRRL Y-2448. The protoplasts were produced by the snail-gut enzyme, and incubation of the protoplasts with labelled glucose and phosphate led to the formation of an exocellular product which was characterized by its hydrolytic properties at pH 2.5. The products of the hydrolyzed polymer had the same Chromatographie characteristics on Sephadex and on paper as the authentic phosphomannan added as carrier.</description><subject>Ascomycota - metabolism</subject><subject>Carbon Isotopes</subject><subject>Chemical Precipitation</subject><subject>Chromatography</subject><subject>Chromatography, Paper</subject><subject>Glucose - metabolism</subject><subject>Glucuronidase</subject><subject>Phosphates - metabolism</subject><subject>Phosphorus Isotopes</subject><subject>Polysaccharides - biosynthesis</subject><subject>Protoplasts - metabolism</subject><subject>Snails</subject><subject>Time Factors</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPwzAQgC0EKqXwD0DKhGAInGPHjwUJVUBBlRiA2XKcq2qUxiVOEP33JG3FyHC64b57fYScU7ihQMUtALBUK0GvhLrWwIVO-QEZU9AiBab4IRn_IcfkJMZPAEq5yEZklAslcqXG5OVtU7dLjD4mYZHgT3BYVV1lm2S9DLGPla1rWyfFJlk3oQ3rysZ2y85sHbHu0WQZqth6f0qOFraKeLbPE_Lx-PA-naXz16fn6f08dSyXbSozdJq5AqVWVDu5KByiYJjlyFFkTInSCpCyYJnVzuV5yQCkkJnjnIMFNiGXu7n9QV8dxtasfBzOtjWGLhrFtZIKBpDvQNeEGBtcmHXjV7bZGApmUGgGP2bwY4QyW4WG920X-_ldscLyr2nvrK_f7erYP_ntsTHReawdlr5B15oy-P8X_AIE6oDv</recordid><startdate>19680101</startdate><enddate>19680101</enddate><creator>Kozak, L.P.</creator><creator>Bretthauer, R.K.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19680101</creationdate><title>Synthesis of exocellular phosphomannan by protoplasts of Hansenula holstii</title><author>Kozak, L.P. ; Bretthauer, R.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-72ec93cbe79819c7fbcee63e25e4e62386da6077b32a9cc55d3007672c4440a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Ascomycota - metabolism</topic><topic>Carbon Isotopes</topic><topic>Chemical Precipitation</topic><topic>Chromatography</topic><topic>Chromatography, Paper</topic><topic>Glucose - metabolism</topic><topic>Glucuronidase</topic><topic>Phosphates - metabolism</topic><topic>Phosphorus Isotopes</topic><topic>Polysaccharides - biosynthesis</topic><topic>Protoplasts - metabolism</topic><topic>Snails</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kozak, L.P.</creatorcontrib><creatorcontrib>Bretthauer, R.K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kozak, L.P.</au><au>Bretthauer, R.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of exocellular phosphomannan by protoplasts of Hansenula holstii</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1968-01-01</date><risdate>1968</risdate><volume>126</volume><issue>3</issue><spage>764</spage><epage>770</epage><pages>764-770</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The biosynthesis of exocellular phosphomannan has been demonstrated with protoplasts of
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| ispartof | Archives of biochemistry and biophysics, 1968-01, Vol.126 (3), p.764-770 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84987800 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Ascomycota - metabolism Carbon Isotopes Chemical Precipitation Chromatography Chromatography, Paper Glucose - metabolism Glucuronidase Phosphates - metabolism Phosphorus Isotopes Polysaccharides - biosynthesis Protoplasts - metabolism Snails Time Factors |
| title | Synthesis of exocellular phosphomannan by protoplasts of Hansenula holstii |
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