Synthesis of exocellular phosphomannan by protoplasts of Hansenula holstii

The biosynthesis of exocellular phosphomannan has been demonstrated with protoplasts of Hansenula holstii NRRL Y-2448. The protoplasts were produced by the snail-gut enzyme, and incubation of the protoplasts with labelled glucose and phosphate led to the formation of an exocellular product which was...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1968-01, Vol.126 (3), p.764-770
Hauptverfasser:	Kozak, L.P., Bretthauer, R.K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ascomycota - metabolism Carbon Isotopes Chemical Precipitation Chromatography Chromatography, Paper Glucose - metabolism Glucuronidase Phosphates - metabolism Phosphorus Isotopes Polysaccharides - biosynthesis Protoplasts - metabolism Snails Time Factors
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Zusammenfassung:	The biosynthesis of exocellular phosphomannan has been demonstrated with protoplasts of Hansenula holstii NRRL Y-2448. The protoplasts were produced by the snail-gut enzyme, and incubation of the protoplasts with labelled glucose and phosphate led to the formation of an exocellular product which was characterized by its hydrolytic properties at pH 2.5. The products of the hydrolyzed polymer had the same Chromatographie characteristics on Sephadex and on paper as the authentic phosphomannan added as carrier.
ISSN:	0003-9861 1096-0384
DOI:	10.1016/0003-9861(68)90469-4