Chromatography of lysosomal enzymes

The soluble fraction of rat liver lysosomes has been chromatographed on DEAE-cellulose, Sephadex G-200, and CM-cellulose. Not all of the lysosomal enzymes measured were separated by chromatography on DEAE-cellulose or Sephadex G-200, although separation into groups of enzymes was possible. CM-cellulose chromatography gave the best resolution. Multiple peaks for acid phosphatase, acid pyrophosphatase, β-acetylglucosaminidase, and ribonuclease were observed and the properties of some of the isoenzymes were studied. Arylsulfatase A and B were well separated. It was confirmed that most of the arylsulfatase in rat liver is of the B type. Many of the enzymes were purified 4- to 18-fold over the soluble fraction of lysosomes. The advantages of fractionating lysosomal enzymes from a single source are discussed.