Optimal conditions for palmitate oxidation by rat heart homogenates
A study of the oxidation of palmitate-1-C 14 to C 14O 2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg 2+. The levels of ATP and Mg 2+ for the optimal rate of palmitate oxidation are interdependen...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1968-10, Vol.128 (1), p.124-128 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Passeron, Susana Savageau, Michael A. Harary, Isaac |
| description | A study of the oxidation of palmitate-1-C
14 to C
14O
2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg
2+. The levels of ATP and Mg
2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg
2+ inhibits the production of CO
2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented. |
| doi_str_mv | 10.1016/0003-9861(68)90014-3 |
| format | Article |
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14 to C
14O
2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg
2+. The levels of ATP and Mg
2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg
2+ inhibits the production of CO
2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(68)90014-3</identifier><identifier>PMID: 5677171</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine Triphosphate - pharmacology ; Animals ; Carbon Dioxide - metabolism ; Carbon Isotopes ; Carnitine - pharmacology ; Coenzyme A - pharmacology ; In Vitro Techniques ; Magnesium - pharmacology ; Methods ; Myocardium - metabolism ; Oxidation-Reduction ; Palmitic Acids - metabolism ; Peroxidases - metabolism ; Rats ; Serum Albumin, Bovine - pharmacology</subject><ispartof>Archives of biochemistry and biophysics, 1968-10, Vol.128 (1), p.124-128</ispartof><rights>1968</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-e5a3c51826e715c0353cae93576f424fc56b40b732f46ec65cc4b24fde2e5a7d3</citedby><cites>FETCH-LOGICAL-c357t-e5a3c51826e715c0353cae93576f424fc56b40b732f46ec65cc4b24fde2e5a7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003986168900143$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5677171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Passeron, Susana</creatorcontrib><creatorcontrib>Savageau, Michael A.</creatorcontrib><creatorcontrib>Harary, Isaac</creatorcontrib><title>Optimal conditions for palmitate oxidation by rat heart homogenates</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>A study of the oxidation of palmitate-1-C
14 to C
14O
2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg
2+. The levels of ATP and Mg
2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg
2+ inhibits the production of CO
2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Carbon Dioxide - metabolism</subject><subject>Carbon Isotopes</subject><subject>Carnitine - pharmacology</subject><subject>Coenzyme A - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Magnesium - pharmacology</subject><subject>Methods</subject><subject>Myocardium - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Palmitic Acids - metabolism</subject><subject>Peroxidases - metabolism</subject><subject>Rats</subject><subject>Serum Albumin, Bovine - pharmacology</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAURYMo4zj6DxS6El1Uk-aj7UaQ4hcMzEbXIU1fNdI2NcmI8-9NnWGWbhLIPfeFdxA6J_iGYCJuMcY0LQtBrkRxXWJMWEoP0JzgUqSYFuwQzffIMTrx_jMyhIlshmZc5DnJyRxVqzGYXnWJtkNjgrGDT1rrklF1vQkqQGJ_TKOmIKk3iVMh-QDl4ml7-w5DJPwpOmpV5-Fsdy_Q2-PDa_WcLldPL9X9MtWU5yEFrqjmpMgE5IRrTDnVCsqYiZZlrNVc1AzXOc1aJkALrjWr43sDWazmDV2gy-3c0dmvNfgge-M1dJ0awK69LFgpMC54BNkW1M5676CVo4tLuo0kWE7u5CRGTmKkKOSfO0lj7WI3f1330OxLO1kxv9vmEJf8NuCk1wYGDY1xoINsrPn_g18d333e</recordid><startdate>196810</startdate><enddate>196810</enddate><creator>Passeron, Susana</creator><creator>Savageau, Michael A.</creator><creator>Harary, Isaac</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196810</creationdate><title>Optimal conditions for palmitate oxidation by rat heart homogenates</title><author>Passeron, Susana ; Savageau, Michael A. ; Harary, Isaac</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-e5a3c51826e715c0353cae93576f424fc56b40b732f46ec65cc4b24fde2e5a7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Carbon Dioxide - metabolism</topic><topic>Carbon Isotopes</topic><topic>Carnitine - pharmacology</topic><topic>Coenzyme A - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Magnesium - pharmacology</topic><topic>Methods</topic><topic>Myocardium - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Palmitic Acids - metabolism</topic><topic>Peroxidases - metabolism</topic><topic>Rats</topic><topic>Serum Albumin, Bovine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Passeron, Susana</creatorcontrib><creatorcontrib>Savageau, Michael A.</creatorcontrib><creatorcontrib>Harary, Isaac</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Passeron, Susana</au><au>Savageau, Michael A.</au><au>Harary, Isaac</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimal conditions for palmitate oxidation by rat heart homogenates</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1968-10</date><risdate>1968</risdate><volume>128</volume><issue>1</issue><spage>124</spage><epage>128</epage><pages>124-128</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>A study of the oxidation of palmitate-1-C
14 to C
14O
2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg
2+. The levels of ATP and Mg
2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg
2+ inhibits the production of CO
2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5677171</pmid><doi>10.1016/0003-9861(68)90014-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1968-10, Vol.128 (1), p.124-128 |
| issn | 0003-9861 1096-0384 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - pharmacology Animals Carbon Dioxide - metabolism Carbon Isotopes Carnitine - pharmacology Coenzyme A - pharmacology In Vitro Techniques Magnesium - pharmacology Methods Myocardium - metabolism Oxidation-Reduction Palmitic Acids - metabolism Peroxidases - metabolism Rats Serum Albumin, Bovine - pharmacology |
| title | Optimal conditions for palmitate oxidation by rat heart homogenates |
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