Optimal conditions for palmitate oxidation by rat heart homogenates

A study of the oxidation of palmitate-1-C 14 to C 14O 2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg 2+. The levels of ATP and Mg 2+ for the optimal rate of palmitate oxidation are interdependen...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1968-10, Vol.128 (1), p.124-128
Hauptverfasser:	Passeron, Susana, Savageau, Michael A., Harary, Isaac
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - pharmacology Animals Carbon Dioxide - metabolism Carbon Isotopes Carnitine - pharmacology Coenzyme A - pharmacology In Vitro Techniques Magnesium - pharmacology Methods Myocardium - metabolism Oxidation-Reduction Palmitic Acids - metabolism Peroxidases - metabolism Rats Serum Albumin, Bovine - pharmacology
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Zusammenfassung:	A study of the oxidation of palmitate-1-C 14 to C 14O 2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg 2+. The levels of ATP and Mg 2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg 2+ inhibits the production of CO 2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.
ISSN:	0003-9861 1096-0384
DOI:	10.1016/0003-9861(68)90014-3