Periodontal regeneration using engineered bone marrow mesenchymal stromal cells
Abstract Regeneration of lost periodontium is a challenge in that both hard (alveolar bone, cementum) and soft (periodontal ligament) connective tissues need to be restored to their original architecture. Bone marrow mesenchymal stromal cells (BM-MSCs) appear to be an attractive candidate for connec...
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| Veröffentlicht in: | Biomaterials 2010-11, Vol.31 (33), p.8574-8582 |
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| creator | Yang, Yi Rossi, Fabio M.V Putnins, Edward E |
| description | Abstract Regeneration of lost periodontium is a challenge in that both hard (alveolar bone, cementum) and soft (periodontal ligament) connective tissues need to be restored to their original architecture. Bone marrow mesenchymal stromal cells (BM-MSCs) appear to be an attractive candidate for connective tissue regeneration. We hypothesized that BM-MSCs are able to sense biological cues from the local microenvironment and organize appropriately to contribute to the regeneration of both soft and hard periodontal connective tissues. To test this hypothesis, we transplanted GFP+ rat BM-MSCs expanded ex vivo on microcarrier gelatin beads into a surgically created rat periodontal defect. After three weeks, evidence of regeneration of bone, cementum and periodontal ligament was observed in both transplanted and control animals. However, the animals that received BM-MSCs regenerated significantly greater new bone. In addition, the animals that had received the cells and beads transplant had significantly more appropriately orientated periodontal ligament fibers, indicative of functional restoration. Finally, donor-derived BM-MSCs were found integrated in newly formed bone, cementum and periodontal ligament, suggesting that they can directly contribute to the regeneration of cells of these tissues. |
| doi_str_mv | 10.1016/j.biomaterials.2010.06.026 |
| format | Article |
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Bone marrow mesenchymal stromal cells (BM-MSCs) appear to be an attractive candidate for connective tissue regeneration. We hypothesized that BM-MSCs are able to sense biological cues from the local microenvironment and organize appropriately to contribute to the regeneration of both soft and hard periodontal connective tissues. To test this hypothesis, we transplanted GFP+ rat BM-MSCs expanded ex vivo on microcarrier gelatin beads into a surgically created rat periodontal defect. After three weeks, evidence of regeneration of bone, cementum and periodontal ligament was observed in both transplanted and control animals. However, the animals that received BM-MSCs regenerated significantly greater new bone. In addition, the animals that had received the cells and beads transplant had significantly more appropriately orientated periodontal ligament fibers, indicative of functional restoration. Finally, donor-derived BM-MSCs were found integrated in newly formed bone, cementum and periodontal ligament, suggesting that they can directly contribute to the regeneration of cells of these tissues.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2010.06.026</identifier><identifier>PMID: 20832109</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Acid Phosphatase - metabolism ; Adult bone marrow mesenchymal stromal cells ; Advanced Basic Science ; Animals ; Beads ; Bone marrow ; Bone Marrow Cells - cytology ; Bone Marrow Cells - drug effects ; Bones ; Cementogenesis - drug effects ; Cements ; Connective tissue ; Dental Cementum - cytology ; Dental Cementum - drug effects ; Dentistry ; Gelatin - pharmacology ; Green Fluorescent Proteins - metabolism ; In vivo ; Isoenzymes - metabolism ; Ligaments ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Microspheres ; Osteocalcin - metabolism ; Osteogenesis - drug effects ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Periodontal Ligament - growth & development ; Periodontal regeneration ; Periodontium - drug effects ; Periodontium - pathology ; Periodontium - physiology ; Rats ; Rats, Sprague-Dawley ; Regeneration ; Regeneration - drug effects ; Regeneration - physiology ; Restoration ; Stromal Cells - cytology ; Stromal Cells - drug effects ; Tartrate-Resistant Acid Phosphatase ; Tissue engineering ; Wound Healing - drug effects</subject><ispartof>Biomaterials, 2010-11, Vol.31 (33), p.8574-8582</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-f14ee9f947493560b18ed8a7358af5e8bac2ec66a5086c589d21ab600188b21c3</citedby><cites>FETCH-LOGICAL-c499t-f14ee9f947493560b18ed8a7358af5e8bac2ec66a5086c589d21ab600188b21c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2010.06.026$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20832109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Yi</creatorcontrib><creatorcontrib>Rossi, Fabio M.V</creatorcontrib><creatorcontrib>Putnins, Edward E</creatorcontrib><title>Periodontal regeneration using engineered bone marrow mesenchymal stromal cells</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Regeneration of lost periodontium is a challenge in that both hard (alveolar bone, cementum) and soft (periodontal ligament) connective tissues need to be restored to their original architecture. Bone marrow mesenchymal stromal cells (BM-MSCs) appear to be an attractive candidate for connective tissue regeneration. We hypothesized that BM-MSCs are able to sense biological cues from the local microenvironment and organize appropriately to contribute to the regeneration of both soft and hard periodontal connective tissues. To test this hypothesis, we transplanted GFP+ rat BM-MSCs expanded ex vivo on microcarrier gelatin beads into a surgically created rat periodontal defect. After three weeks, evidence of regeneration of bone, cementum and periodontal ligament was observed in both transplanted and control animals. However, the animals that received BM-MSCs regenerated significantly greater new bone. In addition, the animals that had received the cells and beads transplant had significantly more appropriately orientated periodontal ligament fibers, indicative of functional restoration. Finally, donor-derived BM-MSCs were found integrated in newly formed bone, cementum and periodontal ligament, suggesting that they can directly contribute to the regeneration of cells of these tissues.</description><subject>Acid Phosphatase - metabolism</subject><subject>Adult bone marrow mesenchymal stromal cells</subject><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Beads</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bones</subject><subject>Cementogenesis - drug effects</subject><subject>Cements</subject><subject>Connective tissue</subject><subject>Dental Cementum - cytology</subject><subject>Dental Cementum - drug effects</subject><subject>Dentistry</subject><subject>Gelatin - pharmacology</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>In vivo</subject><subject>Isoenzymes - metabolism</subject><subject>Ligaments</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Microspheres</subject><subject>Osteocalcin - metabolism</subject><subject>Osteogenesis - drug effects</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - drug effects</subject><subject>Periodontal Ligament - growth & development</subject><subject>Periodontal regeneration</subject><subject>Periodontium - drug effects</subject><subject>Periodontium - pathology</subject><subject>Periodontium - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regeneration</subject><subject>Regeneration - drug effects</subject><subject>Regeneration - physiology</subject><subject>Restoration</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - drug effects</subject><subject>Tartrate-Resistant Acid Phosphatase</subject><subject>Tissue engineering</subject><subject>Wound Healing - drug effects</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2LFDEQhoMo7rj6F6Txopceq9KddOJBkPUTFlZQzyGdrhkzdidr0q3MvzfNrCIe1FMR8rz19RZjjxC2CCifHra9j5OdKXk75i2H8gFyC1zeYhtUnaqFBnGbbQBbXmuJ_Izdy_kA5Q0tv8vOOKiGI-gNu3pfssQhhtmOVaI9BUp29jFUS_ZhX1HY-0CUaKj6GKiabErxezVRpuA-H6eiynOKa3Q0jvk-u7MrTdGDm3jOPr1-9fHibX159ebdxYvL2rVaz_UOWyK9023X6kZI6FHRoGzXCGV3glRvHScnpRWgpBNKDxxtL8sESvUcXXPOHp_yXqf4daE8m8nntQMbKC7ZqFa3UrWN-CfZCSmF7hpZyCd_JVF2yFWjEQv67IS6FHNOtDPXyZfdHA2CWU0yB_O7SWY1yYA0xaQifnhTZ-knGn5Jf7pSgJcngMoGv3lKJjtf9k2DT-RmM0T_f3We_5HGjT54Z8cvdKR8iEsKqwZN5gbMh_Vc1mtBAOiUxuYH_46_AQ</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Yang, Yi</creator><creator>Rossi, Fabio M.V</creator><creator>Putnins, Edward E</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QO</scope><scope>P64</scope></search><sort><creationdate>20101101</creationdate><title>Periodontal regeneration using engineered bone marrow mesenchymal stromal cells</title><author>Yang, Yi ; Rossi, Fabio M.V ; Putnins, Edward E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-f14ee9f947493560b18ed8a7358af5e8bac2ec66a5086c589d21ab600188b21c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acid Phosphatase - metabolism</topic><topic>Adult bone marrow mesenchymal stromal cells</topic><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Beads</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bones</topic><topic>Cementogenesis - drug effects</topic><topic>Cements</topic><topic>Connective tissue</topic><topic>Dental Cementum - cytology</topic><topic>Dental Cementum - drug effects</topic><topic>Dentistry</topic><topic>Gelatin - pharmacology</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>In vivo</topic><topic>Isoenzymes - metabolism</topic><topic>Ligaments</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Microspheres</topic><topic>Osteocalcin - metabolism</topic><topic>Osteogenesis - drug effects</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - drug effects</topic><topic>Periodontal Ligament - growth & development</topic><topic>Periodontal regeneration</topic><topic>Periodontium - drug effects</topic><topic>Periodontium - pathology</topic><topic>Periodontium - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regeneration</topic><topic>Regeneration - drug effects</topic><topic>Regeneration - physiology</topic><topic>Restoration</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - drug effects</topic><topic>Tartrate-Resistant Acid Phosphatase</topic><topic>Tissue engineering</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Yi</creatorcontrib><creatorcontrib>Rossi, Fabio M.V</creatorcontrib><creatorcontrib>Putnins, Edward E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Yi</au><au>Rossi, Fabio M.V</au><au>Putnins, Edward E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Periodontal regeneration using engineered bone marrow mesenchymal stromal cells</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>31</volume><issue>33</issue><spage>8574</spage><epage>8582</epage><pages>8574-8582</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Regeneration of lost periodontium is a challenge in that both hard (alveolar bone, cementum) and soft (periodontal ligament) connective tissues need to be restored to their original architecture. Bone marrow mesenchymal stromal cells (BM-MSCs) appear to be an attractive candidate for connective tissue regeneration. We hypothesized that BM-MSCs are able to sense biological cues from the local microenvironment and organize appropriately to contribute to the regeneration of both soft and hard periodontal connective tissues. To test this hypothesis, we transplanted GFP+ rat BM-MSCs expanded ex vivo on microcarrier gelatin beads into a surgically created rat periodontal defect. After three weeks, evidence of regeneration of bone, cementum and periodontal ligament was observed in both transplanted and control animals. However, the animals that received BM-MSCs regenerated significantly greater new bone. In addition, the animals that had received the cells and beads transplant had significantly more appropriately orientated periodontal ligament fibers, indicative of functional restoration. Finally, donor-derived BM-MSCs were found integrated in newly formed bone, cementum and periodontal ligament, suggesting that they can directly contribute to the regeneration of cells of these tissues.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>20832109</pmid><doi>10.1016/j.biomaterials.2010.06.026</doi><tpages>9</tpages></addata></record> |
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| subjects | Acid Phosphatase - metabolism Adult bone marrow mesenchymal stromal cells Advanced Basic Science Animals Beads Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Bones Cementogenesis - drug effects Cements Connective tissue Dental Cementum - cytology Dental Cementum - drug effects Dentistry Gelatin - pharmacology Green Fluorescent Proteins - metabolism In vivo Isoenzymes - metabolism Ligaments Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Microspheres Osteocalcin - metabolism Osteogenesis - drug effects Periodontal Ligament - cytology Periodontal Ligament - drug effects Periodontal Ligament - growth & development Periodontal regeneration Periodontium - drug effects Periodontium - pathology Periodontium - physiology Rats Rats, Sprague-Dawley Regeneration Regeneration - drug effects Regeneration - physiology Restoration Stromal Cells - cytology Stromal Cells - drug effects Tartrate-Resistant Acid Phosphatase Tissue engineering Wound Healing - drug effects |
| title | Periodontal regeneration using engineered bone marrow mesenchymal stromal cells |
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