Effects of Buyang Huanwu Tang Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on the Expression of VEGF and Ki-67 in the Brain Tissue of the Cerebral Ischemia-Reperfusion Model Rat
Objective：To explore the mechanism of Buyang Huanwu Tang （补阳还五汤 Decoction Invigorating Yang for Recuperation） combined with bone marrow mesenchymal stem cells （MSCs） transplantation in protecting nerves of cerebral ischemic injury. Methods： Local cerebral ischemia-reperfusion rat model was establish...
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| Veröffentlicht in: | Journal of Traditional Chinese Medicine 2010-12, Vol.30 (4), p.278-282 |
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| creator | 张运克 韩雪永 车志英 |
| description | Objective：To explore the mechanism of Buyang Huanwu Tang （补阳还五汤 Decoction Invigorating Yang for Recuperation） combined with bone marrow mesenchymal stem cells （MSCs） transplantation in protecting nerves of cerebral ischemic injury. Methods： Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang（补阳还五汤）was administrated. Vascular endothelial growth factor （VEGF） and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method. Results： VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group （P〈0.05）, and with the most strongest effect in the combination group. Conclusion： Buyang Huanwu Tang（补阳还五汤）combined with MSCs transplantation repairs the injured blood vessels and lesion tissues possibly by up-regulation of VEGF and Ki-67 expression. |
| doi_str_mv | 10.1016/S0254-6272(10)60056-8 |
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Methods： Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang（补阳还五汤）was administrated. Vascular endothelial growth factor （VEGF） and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method. Results： VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group （P〈0.05）, and with the most strongest effect in the combination group. Conclusion： Buyang Huanwu Tang（补阳还五汤）combined with MSCs transplantation repairs the injured blood vessels and lesion tissues possibly by up-regulation of VEGF and Ki-67 expression.</description><identifier>ISSN: 0255-2922</identifier><identifier>ISSN: 0254-6272</identifier><identifier>DOI: 10.1016/S0254-6272(10)60056-8</identifier><identifier>PMID: 21287785</identifier><language>eng</language><publisher>China</publisher><subject>Animals ; Bone Marrow Transplantation ; Brain - metabolism ; Brain Ischemia - drug therapy ; Brain Ischemia - genetics ; Brain Ischemia - metabolism ; Brain Ischemia - therapy ; Combined Modality Therapy ; Disease Models, Animal ; Drugs, Chinese Herbal - administration & dosage ; Gene Expression Regulation - drug effects ; Humans ; Ki-67 Antigen - genetics ; Ki-67 Antigen - metabolism ; Mesenchymal Stem Cell Transplantation ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - drug therapy ; Reperfusion Injury - genetics ; Reperfusion Injury - metabolism ; Reperfusion Injury - therapy ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; 因子和 ; 大鼠模型 ; 干细胞移植 ; 脑组织 ; 脑缺血再灌注 ; 血管内皮生长因子 ; 补阳还五汤 ; 骨髓间质干细胞</subject><ispartof>Journal of Traditional Chinese Medicine, 2010-12, Vol.30 (4), p.278-282</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-712e6287255c326ef063d54b8d2fa457bf4ab9d8d4f7b0606c8b65317bcdf85d3</citedby><cites>FETCH-LOGICAL-c443t-712e6287255c326ef063d54b8d2fa457bf4ab9d8d4f7b0606c8b65317bcdf85d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86801X/86801X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21287785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>张运克 韩雪永 车志英</creatorcontrib><title>Effects of Buyang Huanwu Tang Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on the Expression of VEGF and Ki-67 in the Brain Tissue of the Cerebral Ischemia-Reperfusion Model Rat</title><title>Journal of Traditional Chinese Medicine</title><addtitle>Journal of Traditional Chinese Medicine</addtitle><description>Objective：To explore the mechanism of Buyang Huanwu Tang （补阳还五汤 Decoction Invigorating Yang for Recuperation） combined with bone marrow mesenchymal stem cells （MSCs） transplantation in protecting nerves of cerebral ischemic injury. Methods： Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang（补阳还五汤）was administrated. Vascular endothelial growth factor （VEGF） and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method. Results： VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group （P〈0.05）, and with the most strongest effect in the combination group. Conclusion： Buyang Huanwu Tang（补阳还五汤）combined with MSCs transplantation repairs the injured blood vessels and lesion tissues possibly by up-regulation of VEGF and Ki-67 expression.</description><subject>Animals</subject><subject>Bone Marrow Transplantation</subject><subject>Brain - metabolism</subject><subject>Brain Ischemia - drug therapy</subject><subject>Brain Ischemia - genetics</subject><subject>Brain Ischemia - metabolism</subject><subject>Brain Ischemia - therapy</subject><subject>Combined Modality Therapy</subject><subject>Disease Models, Animal</subject><subject>Drugs, Chinese Herbal - administration & dosage</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Ki-67 Antigen - genetics</subject><subject>Ki-67 Antigen - metabolism</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - drug therapy</subject><subject>Reperfusion Injury - genetics</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - therapy</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>因子和</subject><subject>大鼠模型</subject><subject>干细胞移植</subject><subject>脑组织</subject><subject>脑缺血再灌注</subject><subject>血管内皮生长因子</subject><subject>补阳还五汤</subject><subject>骨髓间质干细胞</subject><issn>0255-2922</issn><issn>0254-6272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kd2O0zAQhXMBYpeFRwBZ3AAXAcd_cS9p1P0RWyHtFm4tOxm3hsTJ2olK348Hw2kLkiWPPp05o5mTZW8K_KnAhfj8iAlnuSAl-VDgjwJjLnL5LLtMmOdkQchF9jLGn4lLzuWL7IIURJal5JfZn5W1UI8R9RYtp4P2W3Q7ab-f0Gauq74zzkOD9m7coWXvAa11CP0erSGCr3eHTrfocYQOVdC2aBO0j0Or_ahH13uU3rgDtPo9BIjxSCz6sbq5Rto36KvLRYncSbMMOlUbF-MEs2pmFQQwIU24i_UOOqfzBxgg2Olote4baNGDHl9lz61uI7w-_1fZ9-vVprrN77_d3FVf7vOaMTrmZUFApMXTVWpKBFgsaMOZkQ2xmvHSWKbNopENs6XBAotaGsFpUZq6sZI39Cp7f_IdQv80QRxV52Kd9tYe-ikqyRaMElrwpOQnZR36GANYNQTX6XBQBVZzZuqYmZozm9ExMyVT39vzhMl00Pzv-hdYErw7G-96v31yfquMrn9Z14KigtCSYUr_AlSCoUM</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>张运克 韩雪永 车志英</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101201</creationdate><title>Effects of Buyang Huanwu Tang Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on the Expression of VEGF and Ki-67 in the Brain Tissue of the Cerebral Ischemia-Reperfusion Model Rat</title><author>张运克 韩雪永 车志英</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-712e6287255c326ef063d54b8d2fa457bf4ab9d8d4f7b0606c8b65317bcdf85d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Bone Marrow Transplantation</topic><topic>Brain - metabolism</topic><topic>Brain Ischemia - drug therapy</topic><topic>Brain Ischemia - genetics</topic><topic>Brain Ischemia - metabolism</topic><topic>Brain Ischemia - therapy</topic><topic>Combined Modality Therapy</topic><topic>Disease Models, Animal</topic><topic>Drugs, Chinese Herbal - administration & dosage</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Ki-67 Antigen - genetics</topic><topic>Ki-67 Antigen - metabolism</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - drug therapy</topic><topic>Reperfusion Injury - genetics</topic><topic>Reperfusion Injury - metabolism</topic><topic>Reperfusion Injury - therapy</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>因子和</topic><topic>大鼠模型</topic><topic>干细胞移植</topic><topic>脑组织</topic><topic>脑缺血再灌注</topic><topic>血管内皮生长因子</topic><topic>补阳还五汤</topic><topic>骨髓间质干细胞</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>张运克 韩雪永 车志英</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Traditional Chinese Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>张运克 韩雪永 车志英</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Buyang Huanwu Tang Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on the Expression of VEGF and Ki-67 in the Brain Tissue of the Cerebral Ischemia-Reperfusion Model Rat</atitle><jtitle>Journal of Traditional Chinese Medicine</jtitle><addtitle>Journal of Traditional Chinese Medicine</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>30</volume><issue>4</issue><spage>278</spage><epage>282</epage><pages>278-282</pages><issn>0255-2922</issn><issn>0254-6272</issn><abstract>Objective：To explore the mechanism of Buyang Huanwu Tang （补阳还五汤 Decoction Invigorating Yang for Recuperation） combined with bone marrow mesenchymal stem cells （MSCs） transplantation in protecting nerves of cerebral ischemic injury. Methods： Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang（补阳还五汤）was administrated. Vascular endothelial growth factor （VEGF） and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method. Results： VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group （P〈0.05）, and with the most strongest effect in the combination group. 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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Bone Marrow Transplantation Brain - metabolism Brain Ischemia - drug therapy Brain Ischemia - genetics Brain Ischemia - metabolism Brain Ischemia - therapy Combined Modality Therapy Disease Models, Animal Drugs, Chinese Herbal - administration & dosage Gene Expression Regulation - drug effects Humans Ki-67 Antigen - genetics Ki-67 Antigen - metabolism Mesenchymal Stem Cell Transplantation Random Allocation Rats Rats, Sprague-Dawley Reperfusion Injury - drug therapy Reperfusion Injury - genetics Reperfusion Injury - metabolism Reperfusion Injury - therapy Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism 因子和 大鼠模型 干细胞移植 脑组织 脑缺血再灌注 血管内皮生长因子 补阳还五汤 骨髓间质干细胞 |
| title | Effects of Buyang Huanwu Tang Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on the Expression of VEGF and Ki-67 in the Brain Tissue of the Cerebral Ischemia-Reperfusion Model Rat |
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