Effects of Buyang Huanwu Tang Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on the Expression of VEGF and Ki-67 in the Brain Tissue of the Cerebral Ischemia-Reperfusion Model Rat

Objective：To explore the mechanism of Buyang Huanwu Tang （补阳还五汤 Decoction Invigorating Yang for Recuperation） combined with bone marrow mesenchymal stem cells （MSCs） transplantation in protecting nerves of cerebral ischemic injury. Methods： Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang（补阳还五汤）was administrated. Vascular endothelial growth factor （VEGF） and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method. Results： VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group （P〈0.05）, and with the most strongest effect in the combination group. Conclusion： Buyang Huanwu Tang（补阳还五汤）combined with MSCs transplantation repairs the injured blood vessels and lesion tissues possibly by up-regulation of VEGF and Ki-67 expression.