Glucose-6-phosphate Dehydrogenase Isoenzymes in Human Cell Cultures Determined by Sucrose-Agar Gel and Cellulose Acetate Zymograms
Summary Cell lines and strains were studied with respect to possession of G-6-PD isoenzymes, using cellulose acetate strips and sucrose-agar gel electrophoretic media. The latter provided higher resolution of A and B bands. The A and B types of G-6-PD were found to occur in human, diploid cultured c...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1968-07, Vol.128 (3), p.772-776 |
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| container_end_page | 776 |
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| container_issue | 3 |
| container_start_page | 772 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 128 |
| creator | Peterson, W. D. Stulberg, C. S. Swanborg, N. K. Robinson, A. R. |
| description | Summary
Cell lines and strains were studied with respect to possession of G-6-PD isoenzymes, using cellulose acetate strips and sucrose-agar gel electrophoretic media. The latter provided higher resolution of A and B bands. The A and B types of G-6-PD were found to occur in human, diploid cultured cell populations in accordance with racial origin of the donor; while certain other heteroploid cell lines were found to possess A type G-6-PD irrespective of donor race. |
| doi_str_mv | 10.3181/00379727-128-33119 |
| format | Article |
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Cell lines and strains were studied with respect to possession of G-6-PD isoenzymes, using cellulose acetate strips and sucrose-agar gel electrophoretic media. The latter provided higher resolution of A and B bands. The A and B types of G-6-PD were found to occur in human, diploid cultured cell populations in accordance with racial origin of the donor; while certain other heteroploid cell lines were found to possess A type G-6-PD irrespective of donor race.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-128-33119</identifier><identifier>PMID: 5668122</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Agar ; Cellulose ; Continental Population Groups ; Culture Techniques ; Electrophoresis ; Gels ; Glucosephosphate Dehydrogenase - analysis ; HeLa Cells ; Humans ; Isoenzymes - analysis ; Sucrose</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1968-07, Vol.128 (3), p.772-776</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-cc2e06b3707698f91f7632fbb772eb8fd08b87cdae95d1f654be832a7c9cb3973</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5668122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peterson, W. D.</creatorcontrib><creatorcontrib>Stulberg, C. S.</creatorcontrib><creatorcontrib>Swanborg, N. K.</creatorcontrib><creatorcontrib>Robinson, A. R.</creatorcontrib><title>Glucose-6-phosphate Dehydrogenase Isoenzymes in Human Cell Cultures Determined by Sucrose-Agar Gel and Cellulose Acetate Zymograms</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Cell lines and strains were studied with respect to possession of G-6-PD isoenzymes, using cellulose acetate strips and sucrose-agar gel electrophoretic media. The latter provided higher resolution of A and B bands. The A and B types of G-6-PD were found to occur in human, diploid cultured cell populations in accordance with racial origin of the donor; while certain other heteroploid cell lines were found to possess A type G-6-PD irrespective of donor race.</description><subject>Agar</subject><subject>Cellulose</subject><subject>Continental Population Groups</subject><subject>Culture Techniques</subject><subject>Electrophoresis</subject><subject>Gels</subject><subject>Glucosephosphate Dehydrogenase - analysis</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Isoenzymes - analysis</subject><subject>Sucrose</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtuGzEQRQkjhiMr_oEAAVilo82Hl49SkGLZgIAUtps0BMmdlWTsQyGXxbrMl3tXUlKmGmDm3jszB6GvjN4KptkdpUIZxRVhXBMhGDMXaMYKURAhjfmEZpOATIrP6DqlN0pZobi8QleFlJpxPkN_1nUOXQIiyWHXpcPO9YBXsBvK2G2hdQnwU-qgfR8aSHjf4sfcuBYvoa7xMtd9jmN7BT3EZt9Cif2An3OIU-Ji6yJeQ41dWx4NuR7beBGgn5b8GppuG12TvqDLytUJbs51jl4ffrwsH8nm5_ppudiQIITuSQgcqPRCUSWNrgyrlBS88l4pDl5XJdVeq1A6MEXJKlnce9CCOxVM8MIoMUffT7mH2P3OkHrb7FMY73ItdDlZfW8415yNQn4STn-kCJU9xH3j4mAZtRN4-xe8HcHbI_jR9O2cnn0D5T_LmfQ4vzvNk9uCfetybMdn_5f4AXjHjas</recordid><startdate>196807</startdate><enddate>196807</enddate><creator>Peterson, W. D.</creator><creator>Stulberg, C. S.</creator><creator>Swanborg, N. K.</creator><creator>Robinson, A. R.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196807</creationdate><title>Glucose-6-phosphate Dehydrogenase Isoenzymes in Human Cell Cultures Determined by Sucrose-Agar Gel and Cellulose Acetate Zymograms</title><author>Peterson, W. D. ; Stulberg, C. S. ; Swanborg, N. K. ; Robinson, A. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-cc2e06b3707698f91f7632fbb772eb8fd08b87cdae95d1f654be832a7c9cb3973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Agar</topic><topic>Cellulose</topic><topic>Continental Population Groups</topic><topic>Culture Techniques</topic><topic>Electrophoresis</topic><topic>Gels</topic><topic>Glucosephosphate Dehydrogenase - analysis</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Isoenzymes - analysis</topic><topic>Sucrose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peterson, W. D.</creatorcontrib><creatorcontrib>Stulberg, C. S.</creatorcontrib><creatorcontrib>Swanborg, N. K.</creatorcontrib><creatorcontrib>Robinson, A. R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peterson, W. D.</au><au>Stulberg, C. S.</au><au>Swanborg, N. K.</au><au>Robinson, A. R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucose-6-phosphate Dehydrogenase Isoenzymes in Human Cell Cultures Determined by Sucrose-Agar Gel and Cellulose Acetate Zymograms</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1968-07</date><risdate>1968</risdate><volume>128</volume><issue>3</issue><spage>772</spage><epage>776</epage><pages>772-776</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Cell lines and strains were studied with respect to possession of G-6-PD isoenzymes, using cellulose acetate strips and sucrose-agar gel electrophoretic media. The latter provided higher resolution of A and B bands. The A and B types of G-6-PD were found to occur in human, diploid cultured cell populations in accordance with racial origin of the donor; while certain other heteroploid cell lines were found to possess A type G-6-PD irrespective of donor race.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>5668122</pmid><doi>10.3181/00379727-128-33119</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1968-07, Vol.128 (3), p.772-776 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84922821 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Agar Cellulose Continental Population Groups Culture Techniques Electrophoresis Gels Glucosephosphate Dehydrogenase - analysis HeLa Cells Humans Isoenzymes - analysis Sucrose |
| title | Glucose-6-phosphate Dehydrogenase Isoenzymes in Human Cell Cultures Determined by Sucrose-Agar Gel and Cellulose Acetate Zymograms |
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