Glucose-6-phosphate Dehydrogenase Isoenzymes in Human Cell Cultures Determined by Sucrose-Agar Gel and Cellulose Acetate Zymograms

Summary Cell lines and strains were studied with respect to possession of G-6-PD isoenzymes, using cellulose acetate strips and sucrose-agar gel electrophoretic media. The latter provided higher resolution of A and B bands. The A and B types of G-6-PD were found to occur in human, diploid cultured c...

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Veröffentlicht in:Experimental biology and medicine (Maywood, N.J.) N.J.), 1968-07, Vol.128 (3), p.772-776
Hauptverfasser: Peterson, W. D., Stulberg, C. S., Swanborg, N. K., Robinson, A. R.
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Sprache:eng
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Zusammenfassung:Summary Cell lines and strains were studied with respect to possession of G-6-PD isoenzymes, using cellulose acetate strips and sucrose-agar gel electrophoretic media. The latter provided higher resolution of A and B bands. The A and B types of G-6-PD were found to occur in human, diploid cultured cell populations in accordance with racial origin of the donor; while certain other heteroploid cell lines were found to possess A type G-6-PD irrespective of donor race.
ISSN:0037-9727
1535-3702
1535-3699
DOI:10.3181/00379727-128-33119