EFFECT OF DRYING ON SURVIVAL OF EHRLICH ASCITES CARCINOMA CELLS

Effect of drying on Ehrlich ascites tumor cells was investigated. 1) Some cells survived the process of freeze-drying for about 5 hours (the sample seemed dry and white powdery, its moisture content being 4.8-9.9%) when adequate media (for example, sodium glutamate) for suspension were employed. However, preservation of living cells in the freeze-dried material was unsuccessful up to the present. 2) Optimal concentration of sodium glutamate for lyophilization is considered to be 5 to 10% when the ascites is diluted two-fold with sodium glutamate solution. 3) Since the effect of vacuum itself on survival is also conceivable, drying with a current of nitrogen gas from a bomb and a hot air current from a hair drier was also tried with fairly satisfactory results.