Conformational transition in biological membrane studyed by the spin label method

Conformational transitions in electron transport particles (ETP) are recorded by variations in the electron spin resonance spectra of the spin label (2,2,6,6-tetramethyl -4-piperidone-1-oxyl-4-caprilic ether) anisotropy. The spin is solubilized in ETP membrane and fixed by hydrophobic interactions....

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Veröffentlicht in:Biochemical and biophysical research communications 1968-08, Vol.32 (3), p.421-425
Hauptverfasser: Koltover, V.K., Goldfield, M.G., Hendel, L.Ya, Rozantzev, E.G.
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Sprache:eng
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Zusammenfassung:Conformational transitions in electron transport particles (ETP) are recorded by variations in the electron spin resonance spectra of the spin label (2,2,6,6-tetramethyl -4-piperidone-1-oxyl-4-caprilic ether) anisotropy. The spin is solubilized in ETP membrane and fixed by hydrophobic interactions. Conformational transition takes place after addition of oxidation substrates (succinate, NAD-H 2). Respiratory chain oxidation with ferricyanide restores the correlation time which is the ESR spectrum anisotropy quantitative measure, to its original value.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(68)90678-5