The Use of 32Di-Isopropylfluorophosphate (32DFP) As A Platelet Label: Evidence for Reutilization of This Isotope In Man

Certain aspects of 32DFP metabolism in man have been studied. It is probable that about 10 per cent of an injected dose is metabolized and reappears in the platelet. This affects the platelet decay curve after day 8 and may influence data on red and white cell kinetics obtained with this isotope. Considering the effects of reincorporation of this compound, we have studied twenty normal individuals and have found a mean platelet lifespan of 11.5 days. Statistical analysis supports the concept that the platelet decay curve is linear: platelets are removed from the circulation because of senescence rather than in a random fashion. The literature on human platelet lifespans is reviewed. Most authors support the concept of a linear decay curve, although the evidence is conflicting and opinions are divided.