Multicenter evaluation of a new automated electrochemiluminescence immunoassay for the quantification of testosterone compared to liquid chromatography tandem mass spectrometry
A new electrochemiluminescence testosterone immunoassay (Elecsys® Testo II) was evaluated. Within-run precision, total precision, and method comparisons to the former immunoassay and LC-MS/MS were investigated. Reference intervals were established for adults and children. Imprecision ranged from 0.9...
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| Veröffentlicht in: | Clinical biochemistry 2011-02, Vol.44 (2), p.264-267 |
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| container_title | Clinical biochemistry |
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| creator | Brandhorst, Gunnar Streit, Frank Kratzsch, Jürgen Schiettecatte, Johan Roth, Heinz Jürgen Luppa, Peter B. Körner, Antje Kiess, Wieland Binder, Lutz Oellerich, Michael von Ahsen, Nicolas |
| description | A new electrochemiluminescence testosterone immunoassay (Elecsys® Testo II) was evaluated.
Within-run precision, total precision, and method comparisons to the former immunoassay and LC-MS/MS were investigated. Reference intervals were established for adults and children.
Imprecision ranged from 0.9% to 8.5%. Method comparisons revealed slopes from 0.68 to 1.32 and coefficients of correlation from 0.893 to 0.991.
Improved agreement with LC-MS/MS was demonstrated, especially for female patients. The assay may be a suitable alternative for laboratories with no access to MS techniques. |
| doi_str_mv | 10.1016/j.clinbiochem.2010.09.024 |
| format | Article |
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Within-run precision, total precision, and method comparisons to the former immunoassay and LC-MS/MS were investigated. Reference intervals were established for adults and children.
Imprecision ranged from 0.9% to 8.5%. Method comparisons revealed slopes from 0.68 to 1.32 and coefficients of correlation from 0.893 to 0.991.
Improved agreement with LC-MS/MS was demonstrated, especially for female patients. The assay may be a suitable alternative for laboratories with no access to MS techniques.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/j.clinbiochem.2010.09.024</identifier><identifier>PMID: 20923677</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Biological and medical sciences ; Biological Assay ; Chromatography, Liquid ; ECLIA ; Electrochemiluminescence ; Endocrinology ; Humans ; Immunoassay ; Investigative techniques, diagnostic techniques (general aspects) ; LC-MS/MS ; Mass spectrometry ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Reproducibility of Results ; Tandem Mass Spectrometry ; Testosterone</subject><ispartof>Clinical biochemistry, 2011-02, Vol.44 (2), p.264-267</ispartof><rights>2010 The Canadian Society of Clinical Chemists</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-6597f01f42723953bf76dded9f5aadd674b9906535bce27717472891f58786193</citedby><cites>FETCH-LOGICAL-c406t-6597f01f42723953bf76dded9f5aadd674b9906535bce27717472891f58786193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0009912010004480$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23884967$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20923677$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brandhorst, Gunnar</creatorcontrib><creatorcontrib>Streit, Frank</creatorcontrib><creatorcontrib>Kratzsch, Jürgen</creatorcontrib><creatorcontrib>Schiettecatte, Johan</creatorcontrib><creatorcontrib>Roth, Heinz Jürgen</creatorcontrib><creatorcontrib>Luppa, Peter B.</creatorcontrib><creatorcontrib>Körner, Antje</creatorcontrib><creatorcontrib>Kiess, Wieland</creatorcontrib><creatorcontrib>Binder, Lutz</creatorcontrib><creatorcontrib>Oellerich, Michael</creatorcontrib><creatorcontrib>von Ahsen, Nicolas</creatorcontrib><title>Multicenter evaluation of a new automated electrochemiluminescence immunoassay for the quantification of testosterone compared to liquid chromatography tandem mass spectrometry</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>A new electrochemiluminescence testosterone immunoassay (Elecsys® Testo II) was evaluated.
Within-run precision, total precision, and method comparisons to the former immunoassay and LC-MS/MS were investigated. Reference intervals were established for adults and children.
Imprecision ranged from 0.9% to 8.5%. Method comparisons revealed slopes from 0.68 to 1.32 and coefficients of correlation from 0.893 to 0.991.
Improved agreement with LC-MS/MS was demonstrated, especially for female patients. The assay may be a suitable alternative for laboratories with no access to MS techniques.</description><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Chromatography, Liquid</subject><subject>ECLIA</subject><subject>Electrochemiluminescence</subject><subject>Endocrinology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>LC-MS/MS</subject><subject>Mass spectrometry</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Reproducibility of Results</subject><subject>Tandem Mass Spectrometry</subject><subject>Testosterone</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2OFCEUhYnROD2jr2BwYVxVS1E_FEvTUcdkjBtdEwouNp0CqoEa02_lI0pNt6NLw4Jw891zLvcg9Lom25rU_bvDVk3WjzaoPbgtJaVO-JbQ9gna1ANrKsqb5inaEEJ4xWtKrtB1SofypO3QP0dXlHDa9Ixt0K8vy5StAp8hYriX0yKzDR4HgyX28BPLJQcnM2gME6gcHzzttDjrIZU-Bdg6t_ggU5InbELEeQ_4uEifrbHqUS5DyiEVm-ABq-BmGYtoDniyx8VqrPZxNQo_opz3J5yl1-CwK7I4zQ_ODnI8vUDPjJwSvLzcN-j7xw_fdrfV3ddPn3fv7yrVkj5XfceZIbVpKaMN75rRsF5r0Nx0Umrds3bknPRd040KKGM1axkdeG26gQ19zZsb9PasO8dwXMrswtny32mSHsKSxFA2Wc4wFJKfSRVDShGMmKN1Mp5ETcSalziIf_ISa16CcFHCKL2vLi7L6EA_dv4JqABvLoBMSk4mSq9s-ss1w9DyfuV2Zw7KTu4tRJGUXdPRNpblCR3sf4zzG67Av80</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Brandhorst, Gunnar</creator><creator>Streit, Frank</creator><creator>Kratzsch, Jürgen</creator><creator>Schiettecatte, Johan</creator><creator>Roth, Heinz Jürgen</creator><creator>Luppa, Peter B.</creator><creator>Körner, Antje</creator><creator>Kiess, Wieland</creator><creator>Binder, Lutz</creator><creator>Oellerich, Michael</creator><creator>von Ahsen, Nicolas</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110201</creationdate><title>Multicenter evaluation of a new automated electrochemiluminescence immunoassay for the quantification of testosterone compared to liquid chromatography tandem mass spectrometry</title><author>Brandhorst, Gunnar ; Streit, Frank ; Kratzsch, Jürgen ; Schiettecatte, Johan ; Roth, Heinz Jürgen ; Luppa, Peter B. ; Körner, Antje ; Kiess, Wieland ; Binder, Lutz ; Oellerich, Michael ; von Ahsen, Nicolas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-6597f01f42723953bf76dded9f5aadd674b9906535bce27717472891f58786193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Chromatography, Liquid</topic><topic>ECLIA</topic><topic>Electrochemiluminescence</topic><topic>Endocrinology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>LC-MS/MS</topic><topic>Mass spectrometry</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Reproducibility of Results</topic><topic>Tandem Mass Spectrometry</topic><topic>Testosterone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brandhorst, Gunnar</creatorcontrib><creatorcontrib>Streit, Frank</creatorcontrib><creatorcontrib>Kratzsch, Jürgen</creatorcontrib><creatorcontrib>Schiettecatte, Johan</creatorcontrib><creatorcontrib>Roth, Heinz Jürgen</creatorcontrib><creatorcontrib>Luppa, Peter B.</creatorcontrib><creatorcontrib>Körner, Antje</creatorcontrib><creatorcontrib>Kiess, Wieland</creatorcontrib><creatorcontrib>Binder, Lutz</creatorcontrib><creatorcontrib>Oellerich, Michael</creatorcontrib><creatorcontrib>von Ahsen, Nicolas</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brandhorst, Gunnar</au><au>Streit, Frank</au><au>Kratzsch, Jürgen</au><au>Schiettecatte, Johan</au><au>Roth, Heinz Jürgen</au><au>Luppa, Peter B.</au><au>Körner, Antje</au><au>Kiess, Wieland</au><au>Binder, Lutz</au><au>Oellerich, Michael</au><au>von Ahsen, Nicolas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multicenter evaluation of a new automated electrochemiluminescence immunoassay for the quantification of testosterone compared to liquid chromatography tandem mass spectrometry</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>44</volume><issue>2</issue><spage>264</spage><epage>267</epage><pages>264-267</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>A new electrochemiluminescence testosterone immunoassay (Elecsys® Testo II) was evaluated.
Within-run precision, total precision, and method comparisons to the former immunoassay and LC-MS/MS were investigated. Reference intervals were established for adults and children.
Imprecision ranged from 0.9% to 8.5%. Method comparisons revealed slopes from 0.68 to 1.32 and coefficients of correlation from 0.893 to 0.991.
Improved agreement with LC-MS/MS was demonstrated, especially for female patients. The assay may be a suitable alternative for laboratories with no access to MS techniques.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>20923677</pmid><doi>10.1016/j.clinbiochem.2010.09.024</doi><tpages>4</tpages></addata></record> |
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| subjects | Biological and medical sciences Biological Assay Chromatography, Liquid ECLIA Electrochemiluminescence Endocrinology Humans Immunoassay Investigative techniques, diagnostic techniques (general aspects) LC-MS/MS Mass spectrometry Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reproducibility of Results Tandem Mass Spectrometry Testosterone |
| title | Multicenter evaluation of a new automated electrochemiluminescence immunoassay for the quantification of testosterone compared to liquid chromatography tandem mass spectrometry |
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