Enrichment of Antibody Plaque-Forming Cells of Spleen by Sedimentation at Unit Gravity

Summary Spleen cells from mice immunized with sheep erythrocytes were sedimented at unit gravity in a sucrose gradient. Cells forming hemolytic antibody plaques sedimented more rapidly than the bulk of the nonplaque-forming cells, resulting in a partial separation with enrichments of up to 18-fold o...

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Veröffentlicht in:	Experimental biology and medicine (Maywood, N.J.) N.J.), 1968-02, Vol.127 (2), p.478-481
Hauptverfasser:	Mage, Michael G., Evans, Warren H., Peterson, Elbert A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Formation Erythrocytes - immunology Gravitation Methods Mice Sheep Space life sciences Spleen - cytology Spleen - immunology Sucrose
Online-Zugang:	Volltext
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Zusammenfassung:	Summary Spleen cells from mice immunized with sheep erythrocytes were sedimented at unit gravity in a sucrose gradient. Cells forming hemolytic antibody plaques sedimented more rapidly than the bulk of the nonplaque-forming cells, resulting in a partial separation with enrichments of up to 18-fold over their starting concentration.
ISSN:	0037-9727 1535-3702 1535-3699
DOI:	10.3181/00379727-127-32719