Detection of Phosphatase Activity in Polarized Light Following Glycerophosphate Incubation.
Summary A method of detection of phosphatase activity is described whereby some artefacts of diffusion, adsorption and nonspecific sulfide blackening are dealt with by examination of the original calcium or lead phosphate precipitates in polarized light. This technic demonstrates also the presence o...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1951-06, Vol.77 (2), p.266-269 |
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| Sprache: | eng |
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| container_end_page | 269 |
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| container_issue | 2 |
| container_start_page | 266 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 77 |
| creator | Belanger, Leonard F. |
| description | Summary
A method of detection of phosphatase activity is described whereby some artefacts of diffusion, adsorption and nonspecific sulfide blackening are dealt with by examination of the original calcium or lead phosphate precipitates in polarized light. This technic demonstrates also the presence of natural mineral deposits in undecalcified material. Comparable estimates of the amount of salt deposited is made with the help of a densitometer. |
| doi_str_mv | 10.3181/00379727-77-18745 |
| format | Article |
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A method of detection of phosphatase activity is described whereby some artefacts of diffusion, adsorption and nonspecific sulfide blackening are dealt with by examination of the original calcium or lead phosphate precipitates in polarized light. This technic demonstrates also the presence of natural mineral deposits in undecalcified material. Comparable estimates of the amount of salt deposited is made with the help of a densitometer.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-77-18745</identifier><identifier>PMID: 14854015</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Biochemical Phenomena ; Glycerophosphates ; Old Medline ; Phosphoric Monoester Hydrolases</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1951-06, Vol.77 (2), p.266-269</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-27efb0d9f05c928be293d139c4bed9c099a3ab6cf921b38df369b6820bf8be6e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14854015$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Belanger, Leonard F.</creatorcontrib><title>Detection of Phosphatase Activity in Polarized Light Following Glycerophosphate Incubation.</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
A method of detection of phosphatase activity is described whereby some artefacts of diffusion, adsorption and nonspecific sulfide blackening are dealt with by examination of the original calcium or lead phosphate precipitates in polarized light. This technic demonstrates also the presence of natural mineral deposits in undecalcified material. Comparable estimates of the amount of salt deposited is made with the help of a densitometer.</description><subject>Biochemical Phenomena</subject><subject>Glycerophosphates</subject><subject>Old Medline</subject><subject>Phosphoric Monoester Hydrolases</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1951</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD9PwzAQxS0EoqXwAViQJ7YU_2nieKyAlkqV6AATg2U7lzZVGoc4AZVPj0uD2JhOd_d7T3oPoWtKxpym9I4QLqRgIhIioqmYxCdoSGMeRzyR8hQND__oAAzQhfdbQmgsWHKOBnSSxpOwDdHbA7Rg28JV2OV4tXG-3uhWe8DTcP0o2j0uKrxypW6KL8jwslhvWjxzZek-i2qN5-XeQuPqXgh4UdnO6IPh-BKd5br0cNXPEXqdPb7cP0XL5_nifrqMLOdpGzEBuSGZzElsJUsNMMkzyqWdGMikJVJqrk1ic8mo4WmWh3QmSRkxeYAT4CN0e_StG_fegW_VrvAWylJX4Dqv0pA25owFkB5B2zjvG8hV3RQ73ewVJerQqPptVAmhfhoNmpvevDM7yP4UfYUBGB8Br9egtq5rqhD2H8dvFsmAQg</recordid><startdate>195106</startdate><enddate>195106</enddate><creator>Belanger, Leonard F.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>195106</creationdate><title>Detection of Phosphatase Activity in Polarized Light Following Glycerophosphate Incubation.</title><author>Belanger, Leonard F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-27efb0d9f05c928be293d139c4bed9c099a3ab6cf921b38df369b6820bf8be6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1951</creationdate><topic>Biochemical Phenomena</topic><topic>Glycerophosphates</topic><topic>Old Medline</topic><topic>Phosphoric Monoester Hydrolases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belanger, Leonard F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belanger, Leonard F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Phosphatase Activity in Polarized Light Following Glycerophosphate Incubation.</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1951-06</date><risdate>1951</risdate><volume>77</volume><issue>2</issue><spage>266</spage><epage>269</epage><pages>266-269</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
A method of detection of phosphatase activity is described whereby some artefacts of diffusion, adsorption and nonspecific sulfide blackening are dealt with by examination of the original calcium or lead phosphate precipitates in polarized light. This technic demonstrates also the presence of natural mineral deposits in undecalcified material. Comparable estimates of the amount of salt deposited is made with the help of a densitometer.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>14854015</pmid><doi>10.3181/00379727-77-18745</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1951-06, Vol.77 (2), p.266-269 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84855322 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Biochemical Phenomena Glycerophosphates Old Medline Phosphoric Monoester Hydrolases |
| title | Detection of Phosphatase Activity in Polarized Light Following Glycerophosphate Incubation. |
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