Detection of Phosphatase Activity in Polarized Light Following Glycerophosphate Incubation.

Detection of Phosphatase Activity in Polarized Light Following Glycerophosphate Incubation.

Summary A method of detection of phosphatase activity is described whereby some artefacts of diffusion, adsorption and nonspecific sulfide blackening are dealt with by examination of the original calcium or lead phosphate precipitates in polarized light. This technic demonstrates also the presence o...

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Veröffentlicht in:Experimental biology and medicine (Maywood, N.J.) N.J.), 1951-06, Vol.77 (2), p.266-269
1. Verfasser: Belanger, Leonard F.
Format: Artikel
Sprache:eng
Schlagworte:
Biochemical Phenomena
Glycerophosphates
Old Medline
Phosphoric Monoester Hydrolases
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Zusammenfassung:Summary A method of detection of phosphatase activity is described whereby some artefacts of diffusion, adsorption and nonspecific sulfide blackening are dealt with by examination of the original calcium or lead phosphate precipitates in polarized light. This technic demonstrates also the presence of natural mineral deposits in undecalcified material. Comparable estimates of the amount of salt deposited is made with the help of a densitometer.
ISSN:0037-9727
1535-3702
1535-3699
DOI:10.3181/00379727-77-18745