Separation of isolated Chinese hamster metaphase chromosomes into three size-groups

Separation of isolated Chinese hamster metaphase chromosomes into three size-groups

A method is described for the isolation of metaphase Chinese hamster chromosomes and their separation into three size-groups. DNA extracted from fractionated chromosomes does not show heterogeneity in thermal denaturation temperature or buoyant density. The separation technique has been used to demo...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of molecular biology 1968-02, Vol.32 (1), p.101,IN1,105-104,IN1,112
Hauptverfasser: Mendelsohn, John, Moore, Dorothy E., Salzman, Norman P.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Autoradiography
Carbon Isotopes
Centrifugation, Density Gradient
Chromosomes - analysis
Cricetinae
Culture Techniques
Densitometry
DNA - analysis
DNA - biosynthesis
Floxuridine - metabolism
Hot Temperature
Karyotyping
Mitosis
Proteins - analysis
RNA - analysis
Thymidine - metabolism
Tritium
Uridine - metabolism
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A method is described for the isolation of metaphase Chinese hamster chromosomes and their separation into three size-groups. DNA extracted from fractionated chromosomes does not show heterogeneity in thermal denaturation temperature or buoyant density. The separation technique has been used to demonstrate a high rate of DNA synthesis in small hamster chromosomes during late S phase.
ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(68)90148-4