Concentrations of Metabolic Intermediates in Kidneys of Rats with Metabolic Acidosis

GOODMAN et al. 1 have shown that there is enhanced renal gluconeogenesis in the rat with chronic metabolic acidosis. The enhancement of renal gluconeogenesis with glut-amine, glutamate, α-ketoglutarate and oxaloacetate, but not with fructose or glycerol as substrates, led to the tentative conclusions that in acidosis there is acceleration of one of the steps in the gluconeogenesis pathway distal to oxaloacetate. The results presented here represent an attempt to define that step.