Immunochemical studies of foot-and-mouth disease: II. Characterization of RNA-free viruslike particles

A new antigen of foot-and-mouth disease virus (FMDV) is described. It has a sedimentation rate of 75 S, and a density of 1.31 g/ml. It is noninfectious and appears to be devoid of RNA. It has an estimated diameter of 21 mμ. It fixes complement when used as an antigen in the complement-fixation test...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1968-02, Vol.34 (2), p.269-274
Hauptverfasser: Graves, J.H., Cowan, K.M., Trautman, R.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 274
container_issue 2
container_start_page 269
container_title Virology (New York, N.Y.)
container_volume 34
creator Graves, J.H.
Cowan, K.M.
Trautman, R.
description A new antigen of foot-and-mouth disease virus (FMDV) is described. It has a sedimentation rate of 75 S, and a density of 1.31 g/ml. It is noninfectious and appears to be devoid of RNA. It has an estimated diameter of 21 mμ. It fixes complement when used as an antigen in the complement-fixation test and forms a precipitin line with homotypic serum in agar plates. It differs from the 140 S FMDV antigen in that: (a) it cannot be disrupted to the 12 S subunit at pH 6.0; and (b) it appears to react with specific antibody to the 12 S subunit as well as with specific antibody to the 140 S intact virus. It occurs as approximately 85% of the total complement-fixing activity of one of the four foot-and-mouth disease viruses studied.
doi_str_mv 10.1016/0042-6822(68)90236-5
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_84793342</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0042682268902365</els_id><sourcerecordid>84793342</sourcerecordid><originalsourceid>FETCH-LOGICAL-e176t-4353b39fa143a235e4b65e873b9e673a5959f82a1d9cd94e626fad7d27f63c3b3</originalsourceid><addsrcrecordid>eNo9kVtr3DAQhUVpSDdJ_0EKfirNgza6Wbb6UAhLLoaQQEmehVYasUptayvJC82vj7dZ8jLDcL4ZhnMQOqdkSQmVl4QIhmXL2A_ZXijCuMT1J7SgRElMuKCf0eID-YJOcn4h89w05BgdC6ZqRfkC-W4YpjHaDQzBmr7KZXIBchV95WMs2IwOD3Eqm8qFDCbDz6rrltVqY5KxBVJ4NSXEcc__frjCPgFUu5Cm3Ic_UG1NKsH2kM_QkTd9hq-Hfoqeb66fVnf4_vG2W13dY6CNLFjwmq-58oYKbhivQaxlDW3D1wpkw009f-1bZqhT1ikBkklvXONY4yW38-op-v5-d5vi3wly0UPIFvrejBCnrFvRKM4Fm8FvB3BaD-D0NoXBpH_6YMys_3rXYf52FyDpbAOMFlxIYIt2MWhK9D4IvXdZ712ei_4fhK75G2wvefk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>84793342</pqid></control><display><type>article</type><title>Immunochemical studies of foot-and-mouth disease: II. Characterization of RNA-free viruslike particles</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Graves, J.H. ; Cowan, K.M. ; Trautman, R.</creator><creatorcontrib>Graves, J.H. ; Cowan, K.M. ; Trautman, R.</creatorcontrib><description>A new antigen of foot-and-mouth disease virus (FMDV) is described. It has a sedimentation rate of 75 S, and a density of 1.31 g/ml. It is noninfectious and appears to be devoid of RNA. It has an estimated diameter of 21 mμ. It fixes complement when used as an antigen in the complement-fixation test and forms a precipitin line with homotypic serum in agar plates. It differs from the 140 S FMDV antigen in that: (a) it cannot be disrupted to the 12 S subunit at pH 6.0; and (b) it appears to react with specific antibody to the 12 S subunit as well as with specific antibody to the 140 S intact virus. It occurs as approximately 85% of the total complement-fixing activity of one of the four foot-and-mouth disease viruses studied.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(68)90236-5</identifier><identifier>PMID: 4295913</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antigens - analysis ; Aphthovirus - immunology ; Biological Assay ; Centrifugation, Density Gradient ; Chromatography ; Complement Fixation Tests ; Densitometry ; Immunodiffusion ; Mice ; RNA, Viral - analysis ; Ultracentrifugation</subject><ispartof>Virology (New York, N.Y.), 1968-02, Vol.34 (2), p.269-274</ispartof><rights>1968</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0042682268902365$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4295913$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Graves, J.H.</creatorcontrib><creatorcontrib>Cowan, K.M.</creatorcontrib><creatorcontrib>Trautman, R.</creatorcontrib><title>Immunochemical studies of foot-and-mouth disease: II. Characterization of RNA-free viruslike particles</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>A new antigen of foot-and-mouth disease virus (FMDV) is described. It has a sedimentation rate of 75 S, and a density of 1.31 g/ml. It is noninfectious and appears to be devoid of RNA. It has an estimated diameter of 21 mμ. It fixes complement when used as an antigen in the complement-fixation test and forms a precipitin line with homotypic serum in agar plates. It differs from the 140 S FMDV antigen in that: (a) it cannot be disrupted to the 12 S subunit at pH 6.0; and (b) it appears to react with specific antibody to the 12 S subunit as well as with specific antibody to the 140 S intact virus. It occurs as approximately 85% of the total complement-fixing activity of one of the four foot-and-mouth disease viruses studied.</description><subject>Animals</subject><subject>Antigens - analysis</subject><subject>Aphthovirus - immunology</subject><subject>Biological Assay</subject><subject>Centrifugation, Density Gradient</subject><subject>Chromatography</subject><subject>Complement Fixation Tests</subject><subject>Densitometry</subject><subject>Immunodiffusion</subject><subject>Mice</subject><subject>RNA, Viral - analysis</subject><subject>Ultracentrifugation</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kVtr3DAQhUVpSDdJ_0EKfirNgza6Wbb6UAhLLoaQQEmehVYasUptayvJC82vj7dZ8jLDcL4ZhnMQOqdkSQmVl4QIhmXL2A_ZXijCuMT1J7SgRElMuKCf0eID-YJOcn4h89w05BgdC6ZqRfkC-W4YpjHaDQzBmr7KZXIBchV95WMs2IwOD3Eqm8qFDCbDz6rrltVqY5KxBVJ4NSXEcc__frjCPgFUu5Cm3Ic_UG1NKsH2kM_QkTd9hq-Hfoqeb66fVnf4_vG2W13dY6CNLFjwmq-58oYKbhivQaxlDW3D1wpkw009f-1bZqhT1ikBkklvXONY4yW38-op-v5-d5vi3wly0UPIFvrejBCnrFvRKM4Fm8FvB3BaD-D0NoXBpH_6YMys_3rXYf52FyDpbAOMFlxIYIt2MWhK9D4IvXdZ712ei_4fhK75G2wvefk</recordid><startdate>196802</startdate><enddate>196802</enddate><creator>Graves, J.H.</creator><creator>Cowan, K.M.</creator><creator>Trautman, R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>196802</creationdate><title>Immunochemical studies of foot-and-mouth disease: II. Characterization of RNA-free viruslike particles</title><author>Graves, J.H. ; Cowan, K.M. ; Trautman, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e176t-4353b39fa143a235e4b65e873b9e673a5959f82a1d9cd94e626fad7d27f63c3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Animals</topic><topic>Antigens - analysis</topic><topic>Aphthovirus - immunology</topic><topic>Biological Assay</topic><topic>Centrifugation, Density Gradient</topic><topic>Chromatography</topic><topic>Complement Fixation Tests</topic><topic>Densitometry</topic><topic>Immunodiffusion</topic><topic>Mice</topic><topic>RNA, Viral - analysis</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Graves, J.H.</creatorcontrib><creatorcontrib>Cowan, K.M.</creatorcontrib><creatorcontrib>Trautman, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Graves, J.H.</au><au>Cowan, K.M.</au><au>Trautman, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunochemical studies of foot-and-mouth disease: II. Characterization of RNA-free viruslike particles</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1968-02</date><risdate>1968</risdate><volume>34</volume><issue>2</issue><spage>269</spage><epage>274</epage><pages>269-274</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>A new antigen of foot-and-mouth disease virus (FMDV) is described. It has a sedimentation rate of 75 S, and a density of 1.31 g/ml. It is noninfectious and appears to be devoid of RNA. It has an estimated diameter of 21 mμ. It fixes complement when used as an antigen in the complement-fixation test and forms a precipitin line with homotypic serum in agar plates. It differs from the 140 S FMDV antigen in that: (a) it cannot be disrupted to the 12 S subunit at pH 6.0; and (b) it appears to react with specific antibody to the 12 S subunit as well as with specific antibody to the 140 S intact virus. It occurs as approximately 85% of the total complement-fixing activity of one of the four foot-and-mouth disease viruses studied.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4295913</pmid><doi>10.1016/0042-6822(68)90236-5</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0042-6822
ispartof Virology (New York, N.Y.), 1968-02, Vol.34 (2), p.269-274
issn 0042-6822
1096-0341
language eng
recordid cdi_proquest_miscellaneous_84793342
source MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Animals
Antigens - analysis
Aphthovirus - immunology
Biological Assay
Centrifugation, Density Gradient
Chromatography
Complement Fixation Tests
Densitometry
Immunodiffusion
Mice
RNA, Viral - analysis
Ultracentrifugation
title Immunochemical studies of foot-and-mouth disease: II. Characterization of RNA-free viruslike particles
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-16T14%3A12%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunochemical%20studies%20of%20foot-and-mouth%20disease:%20II.%20Characterization%20of%20RNA-free%20viruslike%20particles&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Graves,%20J.H.&rft.date=1968-02&rft.volume=34&rft.issue=2&rft.spage=269&rft.epage=274&rft.pages=269-274&rft.issn=0042-6822&rft.eissn=1096-0341&rft_id=info:doi/10.1016/0042-6822(68)90236-5&rft_dat=%3Cproquest_pubme%3E84793342%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=84793342&rft_id=info:pmid/4295913&rft_els_id=0042682268902365&rfr_iscdi=true