Visual Pigment Fluorescence

Visual Pigment Fluorescence

The fluorescence of cattle rod outer segments (dried) and of rhodopsin in solution lies in the range of 575 to 600 millimicrons with a quantum efficiency of 0.005 if excitation is in the visible band near 500 millimicrons. The emission is abolished by bleaching at -196° C but can be reversibly regen...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1968-01, Vol.159 (3812), p.312-314
Hauptverfasser: Guzzo, Anthony V., Pool, Gary L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bleaching
Cattle
Emission spectra
Fluorescence
Fluorometry
Impurities
Irradiation
Photoreceptor Cells
Pollutant emissions
Quantum efficiency
Retinal Pigments
Room temperature
Spectrum Analysis
Wavelengths
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Beschreibung
Zusammenfassung:The fluorescence of cattle rod outer segments (dried) and of rhodopsin in solution lies in the range of 575 to 600 millimicrons with a quantum efficiency of 0.005 if excitation is in the visible band near 500 millimicrons. The emission is abolished by bleaching at -196° C but can be reversibly regenerated by irradiation with light of longer wavelength (600 millimicrons). This behavior reflects the known interconversion of rhodopsin to prelumirhodopsin at this temperature.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.159.3812.312