Herpes-Type Virus in Cultured Leukocytes From Chimpanzees
Ten continuous cultures were established from white cells of the peripheral bloods from 8 of 11 chimpanzees. Six of these cultures grew singly and in aggregates unattached to the surfaces of the culture vessel and 4 grew as glass-attached monolayers. Four of the chimpanzees were inoculated with huma...
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Veröffentlicht in: | JNCI : Journal of the National Cancer Institute 1968-01, Vol.40 (1), p.181-192 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Ten continuous cultures were established from white cells of the peripheral bloods from 8 of 11 chimpanzees. Six of these cultures grew singly and in aggregates unattached to the surfaces of the culture vessel and 4 grew as glass-attached monolayers. Four of the chimpanzees were inoculated with human-derived cell lines containing herpes-type particles. Two of the suspension cultures were derived from these chimpanzees and 4 from the uninoculated chimpanzees. Chromosome analyses revealed a modal number of 48 and a chimpanzee karyotype in 5 of the suspension cell lines. The sixth line, derived from an inoculated animal, had a modal number of 47 and a human karyotype. Electron microscopic examination revealed herpes-type particles in all 6 suspension cell lines morphologically identical to those observed in cell cultures derived from Burkitt's lymphoma and human leukemia. During logarithmic growth, the cell doubling time was about 24 hours. Established cell lines susceptible to 3 known herpesviruses showed no cytopathic effect when challenged with materials derived from these suspension cultures. |
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ISSN: | 0027-8874 1460-2105 |
DOI: | 10.1093/jnci/40.1.181 |