Design and Application of a Mevalonate-Responsive Regulatory Protein

Screening with a customized protein: The regulatory protein AraC in Escherichia coli was engineered to respond to the inducer mevalonate and used as an endogenous molecular reporter in library screening for improved inducer production (see picture; PBAD is the promoter). Mutants of a mevalonate‐synt...

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Veröffentlicht in:	Angewandte Chemie (International ed.) 2011-02, Vol.50 (5), p.1084-1086
Hauptverfasser:	Tang, Shuang-Yan, Cirino, Patrick C
Format:	Artikel
Sprache:	eng
Schlagworte:	biosensors biosynthesis Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - metabolism Genes, araC Mevalonic Acid - metabolism Protein Binding protein engineering Protein Engineering - methods Recombinant Proteins - biosynthesis Recombinant Proteins - genetics regulatory proteins synthetic biology
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Beschreibung
Zusammenfassung:	Screening with a customized protein: The regulatory protein AraC in Escherichia coli was engineered to respond to the inducer mevalonate and used as an endogenous molecular reporter in library screening for improved inducer production (see picture; PBAD is the promoter). Mutants of a mevalonate‐synthesis operon were expressed in E. coli, and a variant responsible for a fourfold increase in the mevalonate titer was isolated.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201006083