Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain

Human transducin-like enhancer of split 1 （TLE1） plays crucial roles in a number of developmental processes and is involved in pathogenesis of malignancy tumors. The N-terminal glutamine-rich domain （Q domain） of TLE1 mediates its tetramerization and interactions with different DNA-binding transcription factors to regulate Notch and Wnt signaling pathways. To better understand the molecular mechanism of TLE1＇s functions in these pathways, we cloned, purified, and crystallized the TLE1 Q domain （TLE1-Q）. The crystals belong to space group C2221, with the complete diffraction data of the native and Se-Met TLE1-Q collected to 3.5 and 4.1A resol- utions, respectively. The phasing-solving and model build- ing are in progress.