Subunit Recycling during Translation in a Reticulocyte Cell-free System

Subunit Recycling during Translation in a Reticulocyte Cell-free System

32 P-Labeled reticulocyte polyribosomes were prepared and added to a lysate cell-free system which was linear in protein synthetic activity for 40 min at 31°. All of the large and small ribosomal subunits were exchanging with polyribosomes within the first 3 to 4 min of incubation. There was no det...

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Veröffentlicht in:The Journal of biological chemistry 1970-11, Vol.245 (22), p.6237-6239
Hauptverfasser: Howard, G A, Adamson, S D, Herbert, E
Format: Artikel
Sprache:eng
Schlagworte:
Carbon Isotopes
Cell-Free System
Centrifugation, Density Gradient
Chromatography
Histocytochemistry
Models, Biological
Phosphorus Isotopes
Protein Biosynthesis
Reticulocytes
Ribosomes
RNA - biosynthesis
RNA, Transfer
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Zusammenfassung:32 P-Labeled reticulocyte polyribosomes were prepared and added to a lysate cell-free system which was linear in protein synthetic activity for 40 min at 31°. All of the large and small ribosomal subunits were exchanging with polyribosomes within the first 3 to 4 min of incubation. There was no detectable exchange of large or small ribosomal subunits with monoribosomes.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)62684-5